Background: Hemophilia cannot be diagnosed in most laboratories of economically less-developed countries leading to high mortality and morbidity rates.Aim: A diagnostic tool was established ranging from bleeding assessment and a simple bedside test of mixing venous clotting time (VCT) to ...
Roctavian is approved with the AAV5 DetectCDx, a companion diagnostic test intended to help health care providers identify patients who may benefit from receiving Roctavian to treat hemophilia A. The test was proven effective in detecting pre-existing anti-AAV5 antibodies, ...
The concept of coagulation as a waterfall or cascade, with a series of reactions each impacting the subsequent reaction, has been prevalent for a long time. The fact that fluid-phase reactions are inefficient and that platelets and other cell surfaces provide the anionic phospholipids needed for c...
The fundamental concept of management of a diagnosed or confirmed acute bleeding in hemophilia is to achieve quick and aggressive hemostasis, preferably within two hours of the onset of symptoms and correction of coagulopathy, but these measures should not be delayed even if diagnostic tests are pend...
Diagnostic Testing for Mild Hemophilia A in Patients with Discrepant One-Stage, Two-Stage, and Chromogenic Factor VIII: C Assays. In recent years, there has been greater awareness among hemostasis scientists and clinicians that factor VIII coagulant activity (FVIII:C) measured in cert... Duncan,...
We present a patient with a classic clinical presentation of an acquired inhibitor with a difficult diagnostic dilemma. 2. Case Report A 73-year-old female, without a prior history of bleeding or hemophilia, presented with difficult to control bleeding, large ecchymoses over her body, petechiae...
In the clinical setting, the routine use of cell-free plasma assays, with fibrin formation as an endpoint, has proven utility as a diagnostic tool in identifying severe coagulation abnormalities. But these approaches do not give a detailed picture of the entire coagulation reaction and do not ref...
The concentrations of anti-VSV.G Abs were determined in NHP serum by ELISA, coating with recombinant VSV.G (Alpha Diagnostic Intl.) 1 μg/mL and developing with a HRP conjugated mouse anti-monkey IgG Ab (Southern Biotech 1:10,000). RNA extraction and ddPCR RNA extraction was performed...
Interestingly, no clinical platform based on FC is available for detection of intracellular FVIII, and this assay will open novel diagnostic possibilities as in lectin mannose binding protein 1 (LMAN1) and multiple coagulation factor deficiency protein 2 (MCFD2), where FVIII is produced but cannot...
as well as the need for comparative studies of different population's mutation frequencies, requires recording their population-wide spectrum in mutation databases. These databases are likely to facilitate genetic consultation of patients' families and can help optimize national DNA diagnostic services also...