Human embryonic stem cells (hESCs) derived from the human embryo, and more recently adult dermal fibroblasts induced to be reprogrammed into pluripotent stem cells (hIPCs), represent two potential sources of cells for regenerative cell replacement therapies. However, the fundamental aspects of both ...
Mice homozygous for the autosomal recessive mutation "motheaten" have normal numbers of multipotential hematopoietic stem cells in the bone marrow and spleen as determined by spleen colony assay. Histologic examination shows no qualitative abnormality in morphology of stem cell colonies in recipients of...
RNA-seq analysis reveals altered expression of genes involved in HSC function and cell cycle regulation inHSC and progenitor cells. Thus, thismouse model provides a novel approach for studying the critical role of the FA pathway not only in germ cell development but also in the maintenance of ...
Loss of Cav-1 impairs the clonogenic ability and function of HSCs. (a)In vitroclonogenic potential of LT cells from Cav-1+/+and Cav-1−/−mice (n=3). (b) The percentage of CD45.2+cells in the peripheral blood of irradiated recipient mice 2, 4, 8 and 12 weeks after transplantati...
Aging-induced MCPH1 translocation activates necroptosis and impairs hematopoietic stem cell function DNA damage contributes to the aging of hematopoietic stem cells (HSCs), yet the underlying molecular mechanisms are not fully understood. In this study, we... H He,Y Wang,B Tang,... - 《Nature...
The balance between self-renewal and differentiation of hematopoietic stem cells (HSCs) is strictly regulated to sustain blood production throughout adult life. De novo DNA methyltransferase 3-alpha (DNMT3A) is one of the major epigenetic regulators that is essential for efficient HSC differentiation....
Hematopoietic stem cells transplantation In clinical treatment, using two important basic features of stem cell self-renewal and differentiation, already able to treat multiple diseases, and reconstitute hematopoietic function. Hematopoietic stem cell transplantation is performed by transplanting bone marrow cel...
the hematopoietic stem cells will enter into the proliferation state from the resting state to meet the demand for stimulatinghematopoiesis. HSCs can not only maintain the stability of its quantity through self-renewal but also can function as directionalprogenitor cellsof each line, which then can ...
3、(P1)Combined deletion of Scf from both endothelial cells and perivascular stromal cells caused severe HSC depletion and anaemia. In contrast, conditional deletion of Scf from osteoblasts or haematopoietic cells did not affect HSC frequency or function. This proves that there is a perivascular nich...
To test function of hematopoietic stem cells (HSCs) in vivo in C57BL/6 (B6) and Trp53-deficient (Trp53 null) mice by using two HSC enrichment schemes. Bone marrow (BM) Lin CD41 CD48 CD150 + (signaling lymphocyte activation molecules [SLAM]), Lin CD41 CD48 CD150 (SLAM ) and Lin ...