Hematopoietic stem cells are the most illustrious inhabitants of the bone marrow. Direct visualization of endogenous hematopoietic stem cells in this niche is essential to study their functions. Until recently this was not possible in live animals. Recent studies, using state-of-the-art technologies,...
vascular cells, adipocytes, osteoblasts and osteoclasts, as weft as mesenchymal stem cells and hematopoietic stem cells, it was previously thought that cells within bone marrow solely functioned to regenerate cells within the marrow, as well as aft circulating hematopoietic cells in peripheral blood. ...
Cues extrinsic to stem cells, emanating from the surrounding microenvironment or niche, are important in the maintenance of stem cell self-renewal and differentiation capacity. The existence of a stem cell niche has been demonstrated for a variety of stem cell populations in diverse organisms. The ...
imaging of transplanted hematopoietic stem and progenitor cells (HSPCs) was developed to investigate the relationship between HSPCs and components of their microenvironment in the bone marrow. In particular, it allows a direct observation of the behavior of hematopoietic cells during the first few days...
Homing is the phenomenon where transplanted hematopoietic cells find their way into the recipient’s bone marrow. This article describes a protocol for studying the homing of hematopoietic cells to the relative areas in the bone marrow. By isolating the cell population of interest, staining this pop...
The vast majority of hematopoietic stem cells (HSCs) reside in specialized niches within the bone marrow during steady state, maintaining lifelong blood cell production. A small number of HSCs normally traffic throughout the body; however, exogenous stimuli can enhance their release from the niche ...
Hematopoietic stem cells (HSCs) function as the foundational cells generating all hematopoietic cell types and orchestrating the renewal of the entire blood system. Beyond intrinsic mechanisms, they are subject to modulation by extrinsic factors. Predominately housed in the bone marrow postnatally, HSCs...
Hematopoietic stem cells are a critical sub- population of whole bone marrow in the treat- ment of myocardial infarction. Stem Cell Discovery 2013; 3: 117-126.Davy, P., Brienne Walker, B., Wong, L. and Allsopp, R. (2013) Hematopoietic stem cells are a critical sub-population of whole...
Hematopoietic stem cells (HSCs) in the endosteum of mesoderm-derived appendicular bones have been extensively studied. Neural crest-derived bones differ from appendicular bones in developmental origin, mode of bone formation and pathological bone resorpt
Currently, bone marrow (BM) cells, umbilical cord blood (UCB) cells, and G-CSF-mobilized peripheral blood cells are well established in clinical practice. While BM and UCB-based transplantation can be autologous, allogeneic HSCT has been more common and has been successful in combination with...