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Destabilizing Hl or H2 expressing full-length PrP with a KDEL sequence at the C termi- might favor conversion to a PrP"-like molecule that is insoluble nus in place of the GPI anchor addition signal sequence. To our but not resistant to digestion by proteinase K. Whether this in- ...
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However, the mechanism by which agonist binding and loss of Na+ coordination is associated with the conversion to an active receptor conformation is still not well understood. While rhodopsin and the other visual receptors do not accommodate a Na+ ion in this region, most switch 2 residues ...
Conversion of ONPG was monitored spectrophotometricaly and β-gal. Units were calculated using the following equation: 1000 ∗ [OD420nm − (1.75 × OD550nm)] / [(time of reaction in minutes) ∗ (volume cell suspension in ml) ∗ OD600 nm]. 2.4. MalE complementation assay A set of...
Briefly, n-Hexadecanol (in dry CH2Cl2) was placed on ice and treated with dry DMSO and P2O5. After stirring for 2 h at room temperature, the mixture was cooled down to 0 °C and triethylamine was added dropwise, followed by stirring at room temperature for 1 h. The reactions mixture ...
idahoensis specimen was selected for genome assembly and a portion of the abdominal region of the foot muscle (1.2 g) was removed and shipped on dry ice to the HudsonAlpha Institute for Biotechnology (Birmingham, AL) for total genomic DNA extraction and library preparation. DNA was extracted...
Apply ice or a warm washcloth to the sores to help ease pain. Copy machine fluids Peritonitis May spread (radiate) to the back or below the left shoulder blade MRI of the pelvis Your symptoms last longer than a few days Weakness or numbness of arms, legs, face ...
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