(Fig. 8). For example, when analyzing exposure of segments to HeLa cell cytosol (1) translocators secreted to the media could be associated with the HeLa cell membrane but do not form functionaltranslocons(potential false negative); (2) some bacteria could remain associated with the HeLa ...
HeLa Cell LineTRAP AssayAging is a decelerating unidirectional process of life. Shortening of telomeric DNA, the (TTAGGG)n hexanucleotide repeats, which form the caps at the chromosome ends, is implicated to determine the aging process, and more importantly the healthy lifespan itself. Telomerase,...
37,38,39,40,41. However, cellular heterogeneity and complexity greatly affect biochemical reactions making them differ from those in test tubes42,43,44,45. Thus, it is important to verify results obtained from cell-free systems
Cells taken from her body without her knowledge were used to form the HeLa cell line, which has been used extensively in medical research since that time. Lacks’ case has sparked legal and ethical debates over the rights of an individual to his or her genetic material and tissue. Her ...
" the lawsuit alleged. It has beenpreviously reported thatThermo Fisher Scientific said they generate about $35 billion in annual revenue. In the lawsuit, Lacks' estate asked that the company "disgorge the full amount of its net profits obtained by commercializing the HeLa cell line to ...
Full size image Effect of cell surface GAGs digestion on adherence To investigate further the adherence of Lv72 to the GAGs, cell surface GAGs were removed by digestion with bacterial lyases, and the effect of this treatment on the binding of the bacteria was determined. Treatment with chondroiti...
1). Finally, purified IgG from SJK 132-20, a monoclonal cell line producing neutralizing antibody against KB cell pol α, confirmed the separation of HeLa pols α and ∊. One hundred to 200 ng of the IgG gave 50% inhibition for 0.06 unit of pol α, and additional antibody neutralized ...
(AML)10, while, in glioblastoma cells, the presence of glycosylated NCL at the cell surface increases with the malignancy of the tumor11. The presence of this extracellular form of NCL seems to be a hallmark of proliferative and cancer cells5and is now the target of several molecules that ...
Full size image Cell culture Hela, a human cervical cancer cell line, was obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA) and maintained in DMEM medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 U/ml streptomycin. The cells were main...
Soluble guanylyl cyclase (sGC) is a heterodimeric enzyme constituted by two subunits, α1 and β1. Previously we have shown that 17β-estradiol (E2) exerts opposite effects on these subunits by increasing α1 and decreasing both β1 expression and enzyma