hTLR4Cells(3-7x10 6 cells)infreezingmedium IMPORTANT:Cellsareshippedfrozen.Ifcellsarenotfrozenupon arrival,contactInvivoGenimmediately. •2x1mlHEK-Blue ™ Selection(250Xconcentrate).Asolution containingseveralselectionantibiotics.HEK-Blue ™ Selectioncanbe storedat4°Cfor6monthsorat-20°Cfor12month...
HEK-Blue™ hTLR4 cells express the human TLR4, MD-2 and CD14 co-receptor genes, and an inducible SEAP reporter gene. They are designed for studying the stimulation of TLR4 by monitoring the activation of NF-kB/AP1. Functionally tested and guaranteed my
mTLR4Cells(5-7x10 6 cells)inFreezingMedium. IMPORTANT:Cellsareshippedfrozen.Ifcellsarenotfrozenupon arrival,contactInvivoGenimmediately. •2mlHEK-Blue ™ Selection(250X).Asolutioncontainingseveral selectionantibiotics.HEK-Blue ™ Selectioncanbestoredat4°Cfor6 ...
When acclimating a cell line to suspension culture in preparation for scale-up to a stirred-tank bioreactor, it is important to confrm that protein expression was not impacted by the adaptation process. Whole cell lysates from untransfected 293 and 293/hTLR4-HA were analyzed for hTLR4-HA exp...
Figure 3:hTLR4-HA expression in untransfected (A) and transfected (B, C) 293 cells. In all panels, hTLR4-HA is detected in red and DAPI in blue. A and B: Photographed at 200 X magnifcation, scale bar = 200 µm. Panel C: Photographed at 400 X, scale bar = 50 µm. ...
Figure 3:hTLR4-HA expression in untransfected (A) and transfected (B, C) 293 cells. In all panels, hTLR4-HA is detected in red and DAPI in blue. A and B: Photographed at 200 X magnifcation, scale bar = 200 µm. Panel C: Photographed at 400 X, scale bar = 50 µm. ...
HEK-Blue™ mTLR2 cells HEK-Blue™ hTLR4 cells HEK-Blue™ mTLR4 cells HEK-Blue™ IL-1R cells HEK-Blue™ IL-6 cells HEK-Blue™ IL-10 cells HEK-Blue™ IL-11 cells HEK-Blue™ IL-12 cells HEK-Blue™ IL-17 cells HEK-Blue™ IL-17C cells HEK-Blue™ IL-18 cells HE...