Fernandes M., Xiao H., and Lis J.T., 1994, Fine structure analyses of the Drosophila and Saccharomyces heat shock factor--heat shock element interactions, Nucleic Acids Res, 22(2): 167-173 http://dx.doi.org/10.1093/nar/22.2.167
In response to proteotoxic stress, HSF1 trimerizes, accumulates in the nucleus, and binds to DNA sequences known as heat shock elements (HSEs), which consist of inverted repeats of the consensus sequence nGAAn (Fig. 1A) (3, 14). Importantly, although the consensus HSE sequence is well ...
A promoter deletion analysis revealed an incomplete but indispensable HSE fragment upstream of -539 to -523 bp, which was identified as the key cis-acting element of OsHSP16.9 C (Zhang et al. 2016b). Thermotolerant loci Heat tolerance in rice is a very complex quantitative trait that is ...
heat-shock protein HSP70: heat-shock protein 70 CHIP: C-terminus of Hsp70-binding protein HSF1: heat-shock transcription factor 1 HSE: heat-shock element UPS: ubiquitin-proteasome system MEFs: mouse embryonic fibroblasts WT: wild-type KO: knockout RT-PCR: reverse transcription poly...
Genomic footprinting experiments indicate that this lag in response to heat shock is due to the failure of HSF to bind efficiently to the mutated HSE. The rate of response to heat shock of synthetic promoters containing one, two, three, or seven perfect HSEs was similar to that observed for...
panicles and leaves under heat stress, which was similar to its homologous geneAtHSP18.2inArabidopsis. A promoter deletion analysis revealed an incomplete but indispensable HSE fragment upstream of -539 to -523 bp, which was identified as the key cis-acting element ofOsHSP16.9 C(Zhang et al....
网络热激应答元件 网络释义 1. 热激应答元件 ...协调相关基 因的转录。 最常见的应答元件有:热激应答元件(heatshock response element HSE), 糖皮质应答元件(gl… www.docin.com|基于3个网页
e Diagram showing heat shock element (HSE) and non-sense segment (NS) on the promoter of iHSP70. ChIP showing enhanced chromatin binding of RIP140 and HSF1 after As treatment (N = 3 with two repeated tests). Results are presented as means ± SEM, **p < 0.01 compared ...
The binding of heat shock factor (HSF) in response to heat and varying concentrations of bleomycin in the four cell lines was examined using a gel shift assay and a synthetic heat shock element (HSE). Heat (45掳C, 10 min) and exposure to 1 g/ml bleomycin for 1 h at 37掳C induced...
[31]. The primary modulator of HSR is Hsf1p, which recognizes a pentameric heat shock element (HSE) [32] in thepromoter regionsof target genes. Variations of this non-canonical HSE are widespread in the yeast genome [33], which greatly expands possible Hsf1p targets. Interestingly, the ...