Our GWAS for heart shape has provided several interesting candidate genes at signals for PCs 1 and 5 which are not reported for other CV traits. For PC1, signals were found nearVEGFAandVEGFB, two members of the PDGF/VEGF growth factor family, which induce proliferation and migration of vasc...
For RNA sequencing, libraries were built using a TruSeq Stranded mRNA library Preparation Kit (Illumina, USA) following the manufacturer’s protocol. Two runs of RNA sequencing were performed for each library on an Illumina NextSeq 500 platform using single-end 75 bp. The RNA-seq analysis ...
if you emulate the image burned into your mind’s eye: a tightly-composed shot, using a long lens, of the lion with its piercing eyes. We perceive the world with both our optical eye and our mind’s eye. The optical eye scans for important data. Then, the mind...
The following video (courtesy of Dr. Dave Schwartzman, UPMC, Pittsburgh, PA) shows an ex vivo tissue preparation and formation of a steam pop during application of RFA. A significant concern of steam pops is the risk of cardiac perforation. Perforation with tamponade was seen in 1 of 62 (2...
“If Not Now, When? If Not Me, Who?” The Next Move What Are You Doing Here? Keep Your Eyes on The Prize The Unwrapped Christmas Present Brer Rabbit and the Briar Patch “Finding One’s Faith” Open doors "A Pre-Advent Bible Study" ...
For RNA sequencing, libraries were built using a TruSeq Stranded mRNA library Preparation Kit (Illumina, USA) following the manufacturer’s protocol. Two runs of RNA sequencing were performed for each library on an Illumina NextSeq 500 platform using single-end 75 bp. The RNA-seq analysis ...
with a total vascular tissue volume fraction of about 15–20%38. It has been shown using a viable ex vivo preparation that changes in coronary perfusion pressure induced near isotropic-like changes in LV wall deformations as the coronary volume was changed39. Pressure in the coronary artery tree...
[propionate], having a half-maximal effect near∼1 mM propionate (Extended Data Fig.8a). Transcript levels (RT–qPCR) confirmedEgr1downregulation andGsta3upregulation in both male and female PA mice and that the upregulation ofPde9aandMmewas specific to females. Intriguingly, WTMmeandPde9...
Uncropped scans of western blots are shown in Supplementary Fig. 6. Statistical analysis. The sample number for each experiment was determined by a power analysis (http://www.power-analysis.com/) assuming a Cohen's maximum variability of the population mean (determined by variance in published ...
Measurement of HDL, which was found to be a carrier of 3-Cl-Tyr, is possible but requires extensive preparation which limits clinical use. MPO measurement is also influenced by sample storage and time to analysis. Biomarkers of downstream effects of MPO activation are also possible, though they...