Zilurgisertib concentrations in saliva samples were determined using a validated LC-MS/MS method (GLP-142) at Incyte (Wilmington, DE, USA). The plasma, urine, and saliva samples were treated with acetonitrile, vortexed, centrifuged, and diluted with reconstitution solutions prior to injecting to...
Lipopolysaccharide (LPS, endotoxin) was detected in the serum samples with the Limulus amoebocyte lysate test (Charles River, Wilmington, Mass.). E. coli LPS-specific antibodies were determined by ELISA with isotype-specific anti-IgA-, anti-IgM- and anti-IgG-antibody conjugates as described previo...
The human GI microbiome is dynamic and shaped by multiple factors, including the aging process. Previously thought to be sterile until birth, the human microbiome may be seeded in utero [5,6]. The GI microbiome changes rapidly during infancy and early childhood and may be shaped by delivery m...
The DNA concentration was determined by absorbance at 260 nm (A260), and the purity was estimated by determining the A260 to A280 ratio with a Nanodrop spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). Analysis of fecal microbiota by PCR-DGGE Fecal samples from each participant ...
(NanoDrop, Wilmington, DE, USA), and Qubit fluorometer (Life Technologies Corporation, Carlsbad, CA, USA). Both the 16S rRNA gene and WGS libraries were generated and sequenced by the Human Genome Sequencing Center (HGSC) at Baylor College of Medicine. The 16S libraries were generated using ...
(NanoDrop, Wilmington, DE, USA), and Qubit fluorometer (Life Technologies Corporation, Carlsbad, CA, USA). Both the 16S rRNA gene and WGS libraries were generated and sequenced by the Human Genome Sequencing Center (HGSC) at Baylor College of Medicine. The 16S libraries were generated using ...
Alecia Murphy from Wilmington, North Carolina on June 09, 2013: You don't really think about your tongue that much but reading this it makes me realize how important it is. Great hub! Karla Iverson (author) from Oregon on June 07, 2013: Thank you, iguidenetwork! I'm glad you ...
The DNA concentration was determined by absorbance at 260 nm (A260), and the purity was estimated by determining the A260 to A280 ratio with a Nanodrop spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). Analysis of fecal microbiota by PCR-DGGE Fecal samples from each participant ...
(Life Technologies) and pooled equimolarly. The pooled libraries were assessed using an Agilent high sensitivity DNA kit and examined by quantitative PCR (qPCR) using the Kapa Quantification kit for Illumina (Kapa Biosystems, Wilmington, MA, USA) according to the manufacturer’s guidelines. ...
The administration of modified cyclosporine with food has been reported to decrease oral bioavailability. The objective of this study was to compare the pharmacodynamic effects of oral modified cyclosporine in healthy dogs when administered with and with