[7] Weis DD,Wales TE, Engen JR, et al. Identification and characterization of EX1 kineticsin H/D exchange mass spectrometry by peak width analysis. J. Am. Soc. Mass. Spectrom. Springer-Verlag. 2006,17: 1498–1509. [8] Glenn R.Masson, Meredith L. Jenki...
采用基于Fluidigm Helios流式细胞仪(Mass Cytometry Systemfor Single CellAnalysis)用于单细胞质谱分析,该平台能够完成包括单细胞捕获、cDNA合成、实时定量PCR分析、目标区域扩增以及质谱流式细胞分析; (6)基于高精度质谱的免疫多肽组学及免疫学检测平台 基于高精度质谱的免疫多肽组学及免疫学检测平台,能够完成包括多肽分离...
Thus, HDX-(ETD)-MS is clearly a useful and powerful tool in the analysis of protein-drug complexes. Currently, ETD is limited to a targeted approach due to poor sequence coverage and data processing issues.Rebecca J. RoseHannah Maple
HDX-MS Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a technique for studying the conformation of proteins. The principle is that when the protein is immersed in the aqueous solution containing deuterium, the amide hydrogen in the main chain of the protein molecule exchanges with the ...
See how using the Thermo Scientific Orbitrap Fusion Lumos Tribrid MS offers a robust method for the analysis of protein conformation and conformation dynamics.
Gen Webinar Streamlining Glycan Analysis: From Sample Preparation to Data Analysis 7:56 Biosimilar Characterization 1:14:47 Mass Spectrometry – an Essential Tool for Characterization of Monoclonal Antibodies during Development 14:21 Comparison ...
Thus, our HDX-MS analysis provides unique insights into the local conformational dynamics of HA trimers in solution with important implications for HA function. Binding of Fab CR6261 induces changes in the local conformational dynamics of HA that pin- point the epitope. CR6261 is a potent ...
Thus, our HDX-MS analysis provides unique insights into the local conformational dynamics of HA trimers in solution with important implications for HA function. Binding of Fab CR6261 induces changes in the local conformational dynamics of HA that pin- point the epitope. CR6261 is a potent ...
Outline of the HDX-MS simulation workflow and analysis: A set of decoys were first prepared for each protein and the RMSD of each decoy determined by alignment with the native structure. (top row) Five example decoys are shown for alpha lactalbumin along with their corresponding RMSD. (second...
Hydrogen/deuterium exchange mass spectrometry (HDX-MS) is an established method for the interrogation of protein conformation and dynamics. While the data analysis challenge of HDX-MS has been addressed by a number of software packages, new computational tools are needed to keep pace with the impro...