Results HDAC6 ZnF UBP treatment to cells did not affect their viability and resulted in enhanced neurite extension and formation of structures similar to podosomes, lamellipodia and podonuts suggesting the role of this domain in actin re-organization. Also, HDAC6 ZnF UBP treatment caused increase ...
【HDAC6结构及其晶体复合物研究进展】HDAC6主要由NES、DD1、DD2、SE14、ZNF-UBP几部分组成,而抑制剂和HDAC6蛋白的结合模式有单齿和双齿两种。#复合物# #化学# #材料# http://t.cn/A6UZHwFM @中国药科大学
Primary sequence of HDAC6 ubiquitin binding domain (ZnFUBP) is highlighted in blue whereas its three-dimensional structure (PDB ID 3C5K) is represented with cyan ribbons. Sequences corresponding to the tetradecapeptide repeating domain (SE14) and to the nuclear export domains NES1, NES2 are ...
两者的 DD 之间的接头是能够结合动力蛋白和高 亲和力的泛素-结合锌指结构域(ZnF-UBP)。HDAC 的保守的催化结构域含有约 390 个氨基酸残基,形成管状以及更广泛的口袋底部。Zn2+结合在口袋的底部附 近的锌指结构域,在一般情况下,HDAC 抑制剂功能螯合的锌离子,使电荷中继 系统功能失调的。(见图 1)。图 1、HDAC6...
The failure to clear misfolded or aggregated proteins from the cytoplasm of nerve cells and glia is a common pathogenic event in a variety of neurodegenerative disorders. This might be causally related to defects in the major proteolytic systems, i.e., the ubiquitin-proteasomal system and the aut...
The BUZ/Znf-UBP domain is a protein module found in the cytoplasmic deacetylase HDAC6, E3 ubiquitin ligase BRAP2/IMP, and a subfamily of ubiquitin-specific proteases. Although several BUZ domains have been shown to bind ubiquitin with high affinity by recognizing its C-terminal sequence (RLRGG...
HDAC6主要由NES、DD1、DD2、SE14、ZNF-UBP几部分组成,而抑制剂和HDAC6蛋白的结合模式有单齿和双齿两种。doi:10.12677/AMC.2019.73004耿爱新中国药科大学崔昊中国科学院大学张立园中国科学院大学朱雍中国药科大学有机化学教研室陆涛中国药科大学有机化学教研室材料化学前沿...
HDAC6 is the largest protein of the HDAC family with 1215 amino acids, two catalytic domains (CD1 and CD2) and a unique C-terminal zinc-finger ubiquitin-binding domain (ZnF-UBP) [13]. Out of the family of 11 HDAC isozymes, HDAC6 has emerged as a highly attractive and safe target in...
Empty vector, FLAG-tagged full-length HDAC6 (1–1215), DAC1 (1–503), DAC2 (488–834), or ZnF–UBP (835–1215) was co-transfected with Myc-MLH1 in 293T cells. Anti-FLAG immunoprecipitation was performed, followed by Western blotting using anti-Myc and anti-FLAG antibodies (upper and...
Uniquely, HDAC6 contains two functional tandem deacetylase domains, termed DAC1 and DAC2, as well as a ZnF-UBP domain, which is a zinc finger-containing region that is homologous with the noncatalytic domain of several ubiquitin-specific proteases (USPs) (Seigneurin-Berny et al., 2001). The...