We analyzed 30 patients known to be positive with high/intermediate/low HCV RNA load, and kept their serum samples in storage at 4°C for 0, 1, 2, 3, 7, 14, 21 and 28 days. Quantitative detection of HCV RNA was performed by TaqMan real-time PCR. The results showed that no ...
同时抗原消失的时间比RNA晚,所以当HCV RNA检测不到的时候,它也可以用来HCV感染的后期阶段的监测治疗。但是因为HCV cAg与HCV RNA相比,前者的敏感度较低,多项研究表明,该技术在低于5 000～10 000 IU/ml的HCV RNA下不存在反应性[36],在低病毒血...
For quantitative detection of HCV specific RNA using real-time RT-PCR Products Product Details Resources FAQ Products artusHCV RT-PCR Kits CE are intended for in-vitro diagnostic use. artusHCV RG RT-PCR Kit (96) Cat. No. / ID. / ID.4518265 ...
HCV-RNA检测可很好地解决上述问题,HCV-RNA被视为HCV病毒复制的直接最直接、可靠的指标,感染HCV后l～2周即可在血中检出,阳性出现早,“窗口期”短,可以缩短感染后血清阳转前的检测窗口期,是HCV感染的直接依据,对丙肝的早期诊断具有重要意义。而荧光定量逆转录聚合酶链反应(FQ-RT-PCR)是目前检测HCV-RNA最佳的手段[...
目前诊断丙肝的主要病原学指标是HCV RNA、抗-HCV抗体、HCV核心抗原【4】,抗-HCV抗体检测主要用于人群筛查,容易出现假阳性或者假阴性;HCV RNA主要用于确证是否感染了HCV病毒;HCV核心抗原与HCV RNA可同时出现,在HCV RNA检测不及时时作为替代方法【5】。因此,采用PCR的方法检测血清中HCV RNA的病毒拷贝数,可以有效的...
INOGene-Hepatitis C Virus (HCV) Quantitative RT-PCR Kit is an in vitro nucleic acid amplification assay for quantification of Hepatitis C virus (HCV) RNA in human serum or plasma (EDTA) using viral RNA extraction kits via Real-Time PCR instrument. INOGene-Hepatitis C Virus (HCV) Quantitative...
丙型肝炎病毒(HCV)是危害肝脏的主要病毒之一,造成严重的公共卫生问题。目前常用的HCV检测方法有血清学检测和分子生物学检测(HCV-RNA检测),血清学检测又可分为HCV核心抗原(HCV-cAg)检测和HCV抗体(HCV-Ab)检测。 HCV-Ab检测 HCV-Ab是机体由于HCV的刺激而产生的蛋白质,HCV-Ab检测...
丙型肝炎病毒核酸检测试剂盒(PCR-荧光法)COBAS® AmpliPrep/COBAS® TaqMan® HCV Quantitative Test, version 2.0,HCV磁性玻璃颗粒试剂盒、HCV裂解试剂盒、HCV 多元试剂盒、HCV 测试专用试剂盒、HCV 强阳性质控品、HCV 弱阳性质控品、COBAS TaqMan 阴性质控品(人血
Meanwhile, serum HCV-RNA concentration was detected by quantitative PCR. SPSS21.0 and Graphpad Prism 5.0 software were used for data analysis. Analysis of variance (ANOVA) was used for comparison among multi-groups and the t-test was used for comparison between two groups. ResultsThe frequencies ...
目前RQPCR检测HCV RNA在临床已广泛应用,RQPCR技术融合了PCR技术与DNA探针杂交技术的优点,实验的整个过程只在加样时打开1次反应管,在PCR的每个循环中可以直接监测到荧光信号的变化,根据PCR反应酶动力学特点,分析软件会自动对DNA进行定量,因此也有人称RQPCR为实时荧光定量PCR[6](realtime quantitative PCR)。其优点较多...