雅培Real Time HCV 检测是一种应用体外聚合酶链反应（PCR）对丙型肝炎病毒（HCV）感染个体血浆或血细胞中HCV核酸DNA定量检测的方法，基于Abbott m2000 rt 实时荧光定量PCR平台，利用RT-PCR原理定量检测人血清和血浆中HCV DNA。独特的探针设计不但使检测具有极佳的检测性能，而且对各种亚型都有很好的检出率，对于病毒的...
丙型肝炎病毒核酸检测试剂盒(PCR-荧光法)Abbott RealTime HCV,丙型肝炎病毒核酸检测试剂-扩增试剂、丙型肝炎病毒核酸检测试剂-质控品、丙型肝炎病毒核酸检测试剂-校准品。(具体内容详见说明书),丙型肝炎病毒核酸检测试剂盒(PCR-荧光法)Abbott RealTime HCV,<<me_syfw>。
Journal of clinical virology: The official publication of the Pan American Society for Clinical VirologyMallory MA, Lucic D, Ebbert MT, Cloherty GA, Toolsie D. Evaluation of the abbott real time HCV genotype II plus RUO (PLUS) assay with reference to core and NS5B sequencing. J Clin Virol....
HCV-RNA was quantified by means of a commercial real-time RT-PCR assay (RealTime™ HCV, Abbott Molecular Inc, Des Plaines, Il, USA) as specified by the manufacturer. The detection limit was 12 IU/mL. HCV genotypes were determined by VERSANT HCV Genotype 2.0 Assay (LiPa) (Innogenetics,...
目的比较丙型肝炎病毒(hepatitis C virus,HCV)抗原(HCV Ag)与HCV RNA试剂检测HCV感染的性能.方法应用ABBOTT ARCHITECT HCV Ag和Abbott RealTime HCV RNA试剂分别检测经Ortho和DiaSorin公司抗HCV EIA试剂以及CHIRON公司RIBA HCV 3.0 SIA和MP Biomedicals Asia Pacific Pte公司确证试剂检测的304份血浆样本(抗-HCV阳性139...
Methods 355 samples from 286 HCV/HIV co-infected subjects for whom HCV-RNA (Abbott RealTime) was requested were analysed also for HCVAg (Abbott ARCHITECT) in order to evaluate the correlation between the two parameters both in patients treated or untreated for chronic hepatitis C and according ...
Objective Our aims were (i) to determine the success rate of the commercial genotyping assay Abbott RealTime \\{HCV\\} Genotype \\{II\\} at identifying the genotype and the HCV-1 subtype; and (ii) to phylogenetically characterise the obtained indeterminate results. Study design \\{HCV\\}...
目前已在临床广泛应用的2 种高敏HCV RNA 检测方法分别是雅培Abbott RealTime(ART)m2000 PCR 仪(线性范围:12～1×108 IU/mL)和罗氏 COBAS AmpliPrep/COBAS TaqMan2.0(CAP/CTM2)全自动病毒载量仪(线性范围:15～1×108 IU/mL)[11],均可实现较低水平的LOD。高敏HCV RNA 的检测结果可以用4 种不同的方式来...
HCV genotypes were defined using the Abbott RealTime HCV Genotype II assay (Abbott Park, Illinois, U.S.A.). The NS3/NS5B region was sequenced to further subtype HCV strains when the genotype results were ambiguous. Data were analyzed using the Blast program (http://blast.ncbi.nlm.nih.gov...
Plasma HCV-RNA measurements were performed using the Abbott Real-Time HCV Amplification Kit (Abbott Molecular Inc. Des Plaines, IL, USA) according to the manufacturer's instructions. The detection limit was 30 IU/mL, equivalent to 1.48 log10 IU/mL, with the 0.2 mL sample preparation procedure...