HCV-G1 control cells were incubated with DMSO alone (vehicle-treated cells). Cell Viability in Cell Culture The cell viability was assessed by the mitochondrial function, measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte- trazolium bromide (MTT) reduction activity for measuring cell ...
To further confirm this in SUDHL6 cells, lentiviruses encoding full-length NS3/4A, protease domain, helicase domain and its proteolytically inactive form S139A mutant protein were generated. As shown in Figure 2e, the protease domain of NS3/4A protein, but not the protease activity, is ...
6. The HCV nucleic acid of claim 1, 3, or 5 which has the full length sequence as depicted in or corresponding to SEQ ID NO:1. 7. The HCV nucleic acid of claim 1 wherein the 5′-terminal sequence is homologous or complementary to an RNA sequence selected from the group consisting...
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To further confirm this in SUDHL6 cells, lentiviruses encoding full-length NS3/4A, protease domain, helicase domain and its proteolytically inactive form S139A mutant protein were generated. As shown in Figure 2e, the protease domain of NS3/4A protein, but not the protease activity, is ...