目的 探讨升麻中分离的环阿尔廷烷型四环三萜化合物(KY17)对人结肠癌HCT116(p53WT)细胞增殖,凋亡,细胞周期的影响.方法 MTT法测定KY17对小鼠胚胎成纤维细胞(MEF)和HCT116细胞株增殖的影响;检测KY17对HCT116细胞周期的影响;荧光显微镜,流式细胞仪分析细胞凋亡情况;Western blot法检测KY17对细胞凋亡蛋白PARP表达的影响...
C-33A细胞系;细胞传代方法:1:2-1:3传代;每周换液2-3次;细胞形态特性:上皮细胞样;细胞生长特性:贴壁;细胞背景资料:该细胞系由Auersperg N建立,源于宫颈癌活检标本。p53 +,pRB +,在273位密码子发生点突变。乳头状瘤病毒DNA、RNA阴性。 3LL细胞系;细胞传代方法:1:2传代;细胞形态特性:详见细胞说明书;细胞生...
细胞一开始就表现出亚二倍体核型及上皮细胞形态。 连续传代可以观察到核型不稳定。 存在成视网膜细胞瘤蛋白(pRB),但大小不正常。 P53表达上调,且有一个273位密码子的点突变导致Arg -> Cys的替换。 人乳头瘤病毒DNA及RNA阴性。 VP267细胞系;细胞传代方法:1:2-1:3传代;每周换液2-3次;细胞形态特性:详见细胞...
Besides the loss of normal tumor suppressive functions of wt p53, mutant p53 may also display gain-of-function properties that facilitate the promotion of invasion, metastasis, proliferation, and cell survival (Frezza and Martins, 2012, Muller and Vousden, 2013). In fact, tumors harboring p53 ...
Ultraviolet radiation down-regulates expres- sion of the cell-cycle inhibitor p21WAF1/CIP1 in human cancer cells independently of p53. Int J Radiat Biol 1999; 75:301-16 Zang Y, Beard RL, Chandraratna RA, Kang JX. Evidence of a lysosomal pathway for apoptosis induced by the synthetic ...
Our results showed that Chk1 knockdown enhanced the cytotoxicity of LDM through abrogating G2/M arrest and increasing apoptosis to a greater extent in HCT116 p53-/- cells than in p53wt cells. Abrogation of LDM-induced G2/M arrest by Chk1 knockdown was associated with reducing the ...
SW480 (结肠癌细胞,P53+)人 LS174T(结肠癌细胞)人 HCT-8 (回盲肠癌细胞,进口胎牛血清培养)人 HT-29 (结肠癌细胞,进口胎牛血清培养)人 HCT-116 (结肠癌细胞,进口胎牛血清培养)人 HCT-8/VCR(耐长春新碱结肠癌细胞系)人 CaCo-2(结肠癌细胞,进口胎牛血清培养)人 ...
In this context, we firstly elucidated the activity of KATNA1 regulatory regions and endogenous KATNA1 mRNA levels in the presence or absence of p53 using HCT 116 WT and HCT 116 p53 (-/-) cells. Next, we demonstrated the binding of p53 to the KATNA1 promoter and then investigated the ...
Methods: HCT116 human colon cancer cells with wild-type p53 (p53 wt) and HCT116 cells in which p53 was deleted in both alleles (p53 ko) were used. Cells were treated for 4h with recombinant adenovirus expressing IRF-1 (Ad-IRF-1), empty adenoviral vector (蠄5), or mock, and media ...
(p53‐/‐)> HCT116(WT)> HCT116(p21‐/‐);而转染了rps7 siRNA后,rps7基因的表达均明显下调,且迁移能力均受到显著抑制,减幅大小依次为HCT116(p53‐/‐)> HCT116(p21‐/‐)> HCT116(WT).结论 RPS7对结肠癌细胞HCT116迁移运动的促进作用显著,且该作用与细胞内的P53水平有关,提示RPS7可能通过P53通路...