HCT 116 cells are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression. Culture Method Doubling Time 17~48 hrs Methods for Passages Wash by PBS once then 0.05% trypsin-EDTA solution and incubate at room temperature (or at 37ºC), observe...
保存条件:低温避光纯度规格:1x10(6)viable cells/ml 产品类别:有机化学品 "HCT 116 cell line人结肠癌细胞系 细胞背景资料:结肠腺癌;男性 细胞的冻存:目前,细胞冻存最常用的技术是液氮冷冻保存法,主要采用加适量保护剂的缓慢冷冻法冻存细胞。主要操作步骤为:(1)选择处于对数生长期的细胞,在冻存前一天换液。将...
中文名称:HCT 116 epithelioid cells人结肠癌细胞系英文名称:HCT 116 epithelioid cells 保存条件:液氮保存纯度规格:1x10(6)viable cells/ml 产品类别:实验化学品 "HCT 116 epithelioid cells人结肠癌细胞系 细胞背景资料:结肠腺癌;男性 细胞形态:上皮细胞样 ...
In vitro, HCT116 cells grow with a doubling time of approximately 18 hours. They are suitable for in vitro and in vivo experimentation. Immunocompromised mice should be used for in life studies, and will form tumors and metastases following implantation of the cells....
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. ...
HELAHeLaATCCCCL2ATCC,Hela细胞,源于黑人31岁女性,子宫颈腺癌,是一种附着型上皮细胞,要求存于液氮中,Thesecellsareasuitabletransfectionhost,Thiscell
G1 phase cells increased,and S phase cells decreased.The doubling time in blank control group,empty plasmid group and experimental group was (4.718 ± 0.212)h,(4.981 ± 0.24) h,(6.0 ± 0.137) h.The colony number was 121 ± 9.00 in blank control group,117 ± 10.00 in empty plasmid ...
Table 1 Inhibition effects of oxaliplatin on the proliferation of HCT116 cells and HCT116/L-OHP cells detected by MTT (n)3, ±s(D570 nm) Oxal iplatinρB/μg mL-1) HCT1 16 HCT1 16/L-OHP A B C 0 1 70±004 1 30±007 1 33±001 1 78±003 ...
Doubling Time17~48 hrs Methods for PassagesWash by PBS once then 0.05% trypsin-EDTA solution and incubate at room temperature (or at 37ºC), observe cells under an inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes) ...
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. 4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting....