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Equal amounts of protein (40 μg) were firstly separated by 10% SDS-PAGE and transferred onto PVDF membrane. After blocking for nonspecific binding (5% nonfat milk in TBS containing Tween20 (TBS-T)) at room temperature for 1 h, the membranes were incubated with Matrix metallopeptidase ...
Equal amounts of protein (20 μg/lane) were separated on SDS-PAGE and transferred onto a PVDF membrane (Bio-Rad Laboratories, Hercules, CA, USA). The primary antibodies used in this study are displayed in Supplementary Table 1. The membranes were incubated with horseradish peroxidase (HRP)-...
Partial eluent was subjected to SDS-PAGE and Coomassie brilliant blue staining. A strong SIRT7 protein band was visualized, as shown in Figure 1B. According to mass spectrometry (MS) analysis, 15 proteins involved in a variety of biological process, including protein degradation, cell cycle, ...
SDS-polyacrylamide gel electrophoresis (SDS-PAGE) used to separate proteins in each sample, and the proteins were then transferred to the polyvinylidene difluoride membranes (PVDF; Bio-Rad Laboratories, Hercules, CA, USA). The membranes were disposed with 5% non-fat milk and then cultured with ...
After subjecting the collected pellets to SDS-PAGE, the GTP-bound Rab5 and Rab22a in samples were determined by Western blotting. Cell adhesion, proliferation, migration, motility, and invasion assays The processes were performed as previously described10,57. In Transwell motility and invasion ...
In this study, we analyzed the correlation of preoperative serum AFP levels with HCC malignant features and survival after hepatectomy, through 24 months of follow up of 108 patients with HCC, who underwent hepatectomy at a single center.
50 µl of protein A/G PLUS-agarose (Santa Cruz, CA, USA) was added and incubated on a rotating device overnight at 4 °C. Afterward, the mixtures were washed using ice-cold PBS 3 times and boiled with 1 × SDS-PAGE loading buffer, and then the supernatants were utilized for ...