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Partial eluent was subjected to SDS-PAGE and Coomassie brilliant blue staining. A strong SIRT7 protein band was visualized, as shown in Figure 1B. According to mass spectrometry (MS) analysis, 15 proteins involved in a variety of biological process, including protein degradation, cell cycle, ...
Equal amounts of protein (40 μg) were firstly separated by 10% SDS-PAGE and transferred onto PVDF membrane. After blocking for nonspecific binding (5% nonfat milk in TBS containing Tween20 (TBS-T)) at room temperature for 1 h, the membranes were incubated with Matrix metallopeptidase ...