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The importance of HCC codingLori Zimmerman
Full size image EGR1 downregulation prompted HCC cells proliferation in vitro and facilitated tumor growth in vivo To investigate the role of EGR1 in HCC, we conducted an analysis of EGR1 protein expression in both normal liver cell line and HCC cell lines. Our findings revealed that HCC cell...
Full size image We examinedTMEM147expression in 72 HCC tissues and 28 non-tumor liver tissues, and found that the TMEM147 protein staining rate in HCC tissues was 83.3% (60/72) compared to 10.7% (3/28) in normal liver tissues (Fig.1c), and the protein expression level of TMEM147 in...
We found that the expression of METTL1 and WDR4 protein levels were significantly elevated in HCC samples relative to normal liver tissue samples (Additional file 1: Figure S1). The qRT-PCR results showed that METTL1 and WDR4 were significantly upregulated in HCC cells (Fig. 1a), with the...
Full size image BRG1 promotes cell proliferation in vitro and in vivo To investigate the potential functions of BRG1 in hepatic carcinogenesis, we first examined the expression of BRG1 in various HCC cell lines using real-time PCR and western blot analysis. The Huh-7, Li-7 and SMMC-7721 cell...
miRNAs are considered promising non-invasive biomarkers. Serum represents the major source of biomarkers, being readily accessible for many analytical tests. Recently, whole blood has drawn increasing interest in biomarker studies due to the presence of
Long non-coding RNA (lncRNA) plays an important role in gene regulation and cancer development. To explore lncRNA, which is related with FAM and participates in HCC progression, we extracted the sequence results of lncRNA and performed difference analysis and Mfuzz cluster analysis on it (Figure...
You had better be savvy with your codingLori Rousche, MD
Full size image To further confirm the findings from RNA-seq analysis, we examined the levels of cellular 2-NBDG, the indigestible glucose analog as a marker for glycolysis [21,22], in cells from a myriad of models in vitro. Notably, both CCHuh7R and CCPLC-PRF-5R displayed higher 2-...