26(15):3979-3989. DOI: 10.1158/1078-0432.CCR-19-3259. [72] Marofi F, Abdul-Rasheed OF, Rahman HS, et al. CAR-NK cell in cancer immunotherapy; A promising frontier[J]. Cancer Sci, 2021, 112(9):3427-3436....
例如,所述抗体片段可以是Fab,F(ab’)2,Fv或单链Fv(scFv),其中来自H链和L链的Fv片段通过合适的接头连接(Huston等,ProcNatlAcadSciUSA855879-83(1988))。更具体地,抗体片段可用酶如木瓜蛋白酶或胃蛋白酶处理抗体而生成。或者可构建编码该抗体片段的基因,将其插入合适的表达载体,并在适合的宿主细胞中表达(参见,...
[7] Zhou J, Ding T, Pan W, et al. Increased intratumoral regulatory T cells are related to intratumoral macrophages and poor prognosis in hepatocellular carcinoma patients. Int J Cancer, 2009,125:1640-1648. [8] Z...
Huh7 CtrlFlag, and Huh7 CD147Flagcells.BSchematic diagram of periodic administration and monitoring of cells.CWestern blot analysis of p-mTOR, SQSTM1/p62, and LC3 at different time points after treatment with MβCD.D,E,FQuantitation of LC3, SQSTM1/p62, and p-mTOR expression...
F Western blotting was conducted to measure the interfere impact of shRNA on the expression of TH protein in HCC cells. G, H Transwell invasion assays were applied to investigate the impact of suppressing TH on the invasion ability of Huh7, PVTT, as well as MHCC97H cell lines (G). The...
HR-1Cells;细胞背景资料:详见相关文献介绍;细胞传代方法:每2-3天换液;细胞生长特性:悬浮生长 ;细胞形态特性:淋巴母细胞样;相关细胞有:HEK293FCells、Walker-256-TCCells、SNU638细胞 S3 HeLaCells;细胞背景资料:该细胞是1955年由PuckTT,MarcusPI和CieciuraSJ建系的,含HPV-18序列;角蛋白阳性;可用于与染色体突变...
hepg2细胞系购自atcc,并选择用于后续的基因修饰。将细胞常规地在37℃下具有5%co2的加湿温育箱中培养。通过具有10%胎牛血清(fbs,f7524,sigma-aldrich)的rpmi1640培养基(r2405,sigma-aldrich)维持细胞。 为了下调靶标基因pklr、pnpla3和pcsk9,通过沉默子预先设计的sirna试剂盒进行rnai实验(测定id:53用于pklr,121938...
F Transmission electron microscopy observation of the number of mitochondria in CD8+ T cells, with red arrows indicating mitochondria, scale bar=5 μm; G Detection of mtRNA copy numbers in CD8+ T cells by RT-qPCR; H Transmission electron microscopy observation of the number and total length ...
1)准备RPMI-1640培养基(RPMI-1640:GIBCO,货号21875-091),90%;优质胎牛血清,10%。 2)培养条件: 气相:空气,95%;二氧化碳,5%。 温度:37℃,培养箱湿度为70%-80%。 3)冻存液:90%血清,10%DMSO,现用现配,液氮储存。 二.细胞处理: 1)复苏细胞:将含有1mL细胞悬液的冻存管迅速放入 37℃水浴中(水面要低于...
IBMF-7Cells;细胞背景资料:MCF-7细胞保留了多个分化了的乳腺上皮的特性,包括:能通过胞质雌激素受体加工雌二醇并能形成圆形复合物(domes)。该细胞含有Tx-4癌基因。肿瘤坏死因子α(TNFalpha)可以抑制MCF-7细胞的生长。抗雌激素处理细胞能调变IGFBP'S的分泌。;细胞传代方法:1:2传代,3-4天长满;细胞生长特性:贴壁生...