Using dual-luciferase reporter assays, we finally confirmed that miR-505-3p is the critical upstream factor regulating the expression of MELK by binding directly to the 3’ UTR of MELK. miR-505-3p has been reported as an effective tumor inhibitor in multiple cancer types. For example, miR-...
5H). Following this, the dual luciferase reporter assay revealed that EGR1 had a repressive impact on the luciferase activity of the original PFKL promoter, but it had no influence on the luciferase activity of the P2 mutant in the PFKL promoter (Fig. 5I). The aforementioned experimental ...
Shaaban MA, Kamal AM, Faggal SI.Design, synthesis, and biological evaluation of new pyrazoloquinazoline derivatives as dual COX-2/5-LOX inhibitors.Arch Pharm (Weinheim). 2020;353:e2000027. https://doi.org/10.1002/ardp.202000027. Article CAS PubMed Google Scholar Liu L, Zhang P, Zhang ...
Cell competition drives the dominant growth of lenvatinib-resistant cells in HCC To characterize the biological behavior conferred by lenvatinib resistance of HCC cells, we established successfully the lenvatinib-resistant HCC cell strain (Huh7R; IC50 = 40.91 μM) with the resistance characteri...
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Cells were harvested 48 h after transcription and assayed by the Dual Luciferase Reporter Assay System (Promega) using GloMax microplate luminometer (Promega). The TEAD binding domain (TBD) luciferase plasmids and Renilla plasmid was kindly provided by Professor Faxing Yu. Immunohistochemistry Five-...
4a). We generated an oligonucleotide of 23 base pairs containing miR-200b-3p binding sequence of ERG 3′-UTR and cloned into pmirGLO Dual-Luciferase miRNA Target Expression Vector and analyzed if ERG is a direct target of miR-200b-3p by using the dual-luciferase reporter assay. A similar ...