conjugated affinipure goat anti-mouse IgG (H+L) (SA00001-1) and HRP-conjugated affinipure goat anti-rabbit IgG (H+L) (SA00001-2; both Proteintech); and goat anti-mouse IgG (H+L) (Alexa Fluor 568; ab175473) and goat anti-rabbit IgG (H+L) (Alexa Fluor 488; ab150077; both Abcam...
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Crosslinks between SctSpBpa and SctUFLAG in the SctRSTU assembly intermediate and in the assembled needle complex (NC) are indicated. d Immunodetection of the early T3SS substrate SctP on western blottings of SDS-PAGE-separated culture supernatants of the indicated S. typhimurium SctS pBpa ...
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