The 16L2 protein could form a complex with a nuclear protein(s) or could interact with the negatively charged phosphate groups of the DNA backbone via the positively charged arginine residues. Although we have
DNA fragments will "run" toward the electrode during gel electrophoresis because the groups make it a negatively charged molecule. (a) True (b) False. Why does RNA appear on gel electrophoresis as a smear between 28s and 18s? Why do you need to use a buffe...
Metabolite carriers like the ADP/ATP carrier or the phosphate carrier are hydrophobic proteins with 6 transmembrane helices [6]. In contrast to mitochondrial precursor proteins with a cleavable N-terminal presequence, carrier proteins contain hydrophobic, hydrophilic, charged or uncharged sequence motifs...
aThe thermally less stable heteroduplexes denature more extensively and, therefore, are retained a shorter time on the stationary phase. The eluent contains the cationic TEAA ion (0.1 M), which interacts with the negatively charged phosphate groups on DNA molecules and also with the hydrophobic su...
Which component of a cell prevents water from entering the cell? a) The polar phosphate groups b) The transport proteins c) The non-polar lipid bilayer d) The nucleic acids The phospholipids of the plasma membrane are: a. hydrophobic b. hydrophilic c. both hydrophobic...
The buffer reference spectra (250 mM NaF in 50 mM sodium phosphate buffer, pH7) were used as baselines and were subtracted from the CD spectra of the samples. Signal intensities were expressed in millidegrees [θ] and converted to the mean molar ellipticity per residue [θ]MRE in (...
A) Predicted potential sites for phosphate modification on Ser and Thr residues. The light gray horizontal line indicates the threshold for modification potential. The blue, green and red vertical lines showed the potential phosphorylated Ser, Thr and Tyr residues, respectively. B) Predicted potential...
A) Predicted potential sites for phosphate modification on Ser and Thr residues. The light gray horizontal line indicates the threshold for modification potential. The blue, green and red vertical lines showed the potential phosphorylated Ser, Thr and Tyr residues, respectively. B) Predicted potential...
For the preparation of whole cell extracts, cells were harvested by trypsinization and centrifuged at 1000 rpm for 5 min at RT. The supernatant was removed and the pellet was washed once with ice-cold phosphate-buffered saline (PBS) and then centrifuged again. Cell pellet was re-suspended...
Protecting groupsReductionNitrationA 7-nitroindolin-1-yl amide of the neuroactive amino acid 纬-aminobutryate (GABA) has been synthesised with two phosphate groups attached to the indoline nucleus at 4-alkoxy substituent. The compound is designed to release GABA on a sub-microsecond time scale ...