aDue to the negatively charged phosphate groups, the PA tends to chelate with multivalent cations, such as Mg2+, Zn2+, Al3+, etc. 由于消极地被充电的磷酸盐小组, PA倾向于结为螯合物与多值性正离子,例如Mg2+、Zn2+、Al3+等等。[translate] ...
aThe thermally less stable heteroduplexes denature more extensively and, therefore, are retained a shorter time on the stationary phase. The eluent contains the cationic TEAA ion (0.1 M), which interacts with the negatively charged phosphate groups on DNA molecules and also with the hydrophobic su...
Transmissible gastroenteritis virus (TGEV) causes high mortality in young piglets (< 3 days of age). With aging, the susceptibility/morbidity/mortality rates drop. We previously hypothesized that the age-related changes in the intestinal mucus coul
Question: Fill in the blank: The chemical element ___ has a role in both transfers of energy as well as in the structure of nucleic acids. Nucleotides: In chemistry, nucleotides are described as biological organic molecules composed of a n...
Protecting groupsReductionNitrationA 7-nitroindolin-1-yl amide of the neuroactive amino acid 纬-aminobutryate (GABA) has been synthesised with two phosphate groups attached to the indoline nucleus at 4-alkoxy substituent. The compound is designed to release GABA on a sub-microsecond time scale ...
The buffer reference spectra (250 mM NaF in 50 mM sodium phosphate buffer, pH7) were used as baselines and were subtracted from the CD spectra of the samples. Signal intensities were expressed in millidegrees [θ] and converted to the mean molar ellipticity per residue [θ]MRE in (...
Membranes were stripped at 65 °C for 15 min in 50 mm Tris-HCl, 0.2 m 2-mercaptoethanol, and 2% SDS; they were then reprobed with an anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (Abcam) to serve as a protein loading control. Electron Microscopy—Isolated peritoneal MCs ...
For the preparation of whole cell extracts, cells were harvested by trypsinization and centrifuged at 1000 rpm for 5 min at RT. The supernatant was removed and the pellet was washed once with ice-cold phosphate-buffered saline (PBS) and then centrifuged again. Cell pellet was re-suspended...
A) Predicted potential sites for phosphate modification on Ser and Thr residues. The light gray horizontal line indicates the threshold for modification potential. The blue, green and red vertical lines showed the potential phosphorylated Ser, Thr and Tyr residues, respectively. B) Predicted potential...
(ANOVA) with the Bonferroni post hoc test was used. *p< 0.05 and **p< 0.01.Gapdh,glyceraldehyde 3-phosphate dehydrogenase. RQ, relative quantity. (B) The accumulation of keratan sulfate (KS) in brown counterstained with hematoxylin. Arrows indicate intense accumulation, each section is 7 μm...