DNA fragments will "run" toward the electrode during gel electrophoresis because the groups make it a negatively charged molecule. (a) True (b) False. Why does RNA appear on gel electrophoresis as a smear between 28s and 18s? Why do you need to use a buffe...
aDue to the negatively charged phosphate groups, the PA tends to chelate with multivalent cations, such as Mg2+, Zn2+, Al3+, etc. 由于消极地被充电的磷酸盐小组, PA倾向于结为螯合物与多值性正离子,例如Mg2+、Zn2+、Al3+等等。[translate] ...
Since nucleic acids have charged phosphate groups, DNA and RNA contain lots of negative charges. Would you suspect that DNA and RNA are soluble in polar solvent like water? When 10,000 or more amino acids are linked together, what could ...
aThe thermally less stable heteroduplexes denature more extensively and, therefore, are retained a shorter time on the stationary phase. The eluent contains the cationic TEAA ion (0.1 M), which interacts with the negatively charged phosphate groups on DNA molecules and also with the hydrophobic su...
The 16L2 protein could form a complex with a nuclear protein(s) or could interact with the negatively charged phosphate groups of the DNA backbone via the positively charged arginine residues. Although we have previously shown that the NRS of HPV16 L2 can bind promiscuously to DNA in vitro (...
Blots were re-probed for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control. The results are representative of three independent experiments, as described under “Experimental procedures.” Bar charts under A–C showed the relative density of the cleavage product of DrIL-1β in ...
The buffer reference spectra (250 mM NaF in 50 mM sodium phosphate buffer, pH7) were used as baselines and were subtracted from the CD spectra of the samples. Signal intensities were expressed in millidegrees [θ] and converted to the mean molar ellipticity per residue [θ]MRE in (...
For the preparation of whole cell extracts, cells were harvested by trypsinization and centrifuged at 1000 rpm for 5 min at RT. The supernatant was removed and the pellet was washed once with ice-cold phosphate-buffered saline (PBS) and then centrifuged again. Cell pellet was re-suspended...
Which component of a cell prevents water from entering the cell? a) The polar phosphate groups b) The transport proteins c) The non-polar lipid bilayer d) The nucleic acids The phospholipids of the plasma membrane are: a. hydrophobic b. hydrophilic c. both hydrophobic...
Protecting groupsReductionNitrationA 7-nitroindolin-1-yl amide of the neuroactive amino acid 纬-aminobutryate (GABA) has been synthesised with two phosphate groups attached to the indoline nucleus at 4-alkoxy substituent. The compound is designed to release GABA on a sub-microsecond time scale ...