A ‘TATA’-like sequence was found 389 bp upstream from the initial Met codon. No polyadenylation signal was found in the 3' non-coding region. Southern blot analyses indicated that γ-tub is a single-copy gene. Northern blot analyses indicated that a 1.81-kb RNA species was transcribed. ...
The nucleic acid sequence of the cloned region contained an open reading frame which encoded a protein whose size, amino acid composition, and N-terminal sequence corresponded to those of the purified GS. glnB had a high G + C content and codon usage typical of streptomycete genes. A ...
Baculoviruses were generated and amplified using Sf21 cells; and were used to infect High Five cells at a density of 1.5–2.0 million cells per mL to express the recombinant proteins. The conditioned media of High Five cells were collected 2 days after for protein purification. Codon-optimized ...
What is the start codon and what amino acid does it code for? Which three amino acids are often phosphorylated? Give a brief example to your answer. What is a promoter? a. An AT rich region in the DNA sequence that is found in every organism b. The +1 transcription start site where...
Aphids are common crop pests. These insects reproduce by facultative parthenogenesis involving several rounds of clonal reproduction interspersed with an occasional sexual cycle. Furthermore, clonal aphids give birth to live young that are already pregna
Describe the difference between a gene and a codon. Charles has been told that hemophilia runs in their genes. Whose ancestor should he investigate - his mother's or his father's? Why? What is a conduit between two bacteria for gene exchange called?
aThis type of jig does not normally contain any device for clamping of securing the workpiece ,but relies 夹具的这个类型通常不包含任何设备为夹紧巩固制件,而是依靠[translate] ait is possible to allow each codon position to have a different substitution rate, a different amount of rate heterogeneity...
C0380: Clinical and Biochemical Consequences of Met1Ileu Mutation in Serpinc1 Gene: Generation of a Small Non-Inhibitory Antithrombin Variant without the N-Terminal Region by Use of an Alternative Initiation Codon that Has a Strong Gain-......
There is a region in the 5'-end (nt -26 to -12, where +1 is the first nucleotide in the translation initiation codon) that contains a long stretch of thymine (T) bases (15 or 16 Ts). Removal of one thymine (15 Ts) results in a truncation of the 5'-end. Therefore, to ...
The oricoEnvΔ659–699 mutant was created by insertion of a stop codon after the threonine at position 658 to increase incorporation of Env into lentiviral particles. Since the SIV LTR activity that drives the luciferase reporter expression in transduced cells is cell type dependent, we included ...