通过透析(Thermo FisherScientific Inc., Slide-A-Lyzer Dialysis Cassette)用PBS替代级分中的缓冲液,且然后用5 mM磷酸钠/50 mM MES, pH 7.0的缓冲液稀释5倍。将Fc片段溶液施加至用5 mMNaPi/50 mM MES/30 mM NaCl, pH 7.0的缓冲液平衡的陶瓷羟基磷灰石柱(Bio-RadLaboratories, Inc., Bio-Scale CHT l型...