Crystallization experiments with peroxiredoxin of Aeropyrum pernix K1 (thioredoxin peroxidase, ApTPx) and hen egg white lysozyme demonstrated that this large-scale hanging-drop method could produce a large-volume crystal very effectively. A neutron diffraction experiment confirmed that an ApTPx crystal (...
containing 0.05MKCl. The purified protein has been concentrated via Centricon‐10 up to 25mg/ml and stored in 20mMTRIS‐HCl buffer at pH = 7.5 and 2mMDTT to maintain the protein in a reducing environment. Crystallization was carried out using the hanging‐drop vapor‐diffusion met...
Hanging drop crystallization method was used to form high purity lysozyme crystals from the embryonic stage. To this end, this work differs from an earlier work of Forsythe et al., who used seed crystals in the size range of 10m–40m for face growth measurements at different pH values. The...
HDP Hanging Drop Crystallization Plate (Swissci) 英文名称: HDP Hanging Drop Crystallization Plate (Swissci) 总访问: 523 国产/进口: 进口 半年访问: 9 产地/品牌: 美国Hampton 产品类别: 分子生物学试剂 规格: HR3-187 最后更新: 2024-12-5 货号: CAS 号: 参考报价: 询价 立即询价 电话咨询 ...
Crystallization and preliminary X-ray analysis of a bacterial lysozyme produced by Crystals of this enzyme have been obtained by the hanging-drop vapour-diffusion method using polyethylene glycol as a precipitant. They belong to the ... T Shiba,S Harada,H Sugawara,... 被引量: 26发表: 2001年...
Crystallization of the LG-vitamin D3 complex was achieved using the hanging-drop vapor-diffusion method at18 °Cwith 2μL hanging drops containing equal amounts of LG-vitamin D3 complex and a reservoir solution (0.1 MHEPES containing1.4 Mtrisodium citrate dehydrate, pH 7.5). 1.2Weiling Niu[...
英文别名: HDP Hanging Drop Crystallization Plate中文名: 中文别名: CBNumber: CB85520835 分子式: 分子量: 0 MOL File: Mol file 化学性质 安全信息 用途 供应商 9 HDP Hanging Drop Crystallization Plate化学性质 安全信息HDP Hanging Drop Crystallization Plate性质、用途与生产工艺 HDP Hanging Drop ...
Simple hanging drop (20 μl) double antibody radioimmunoassay of human IgM, IgG, IgA, IgD and IgE The immune precipitate in the hanging drop was harvested and washed without centrifugation with a simple Terasaki plate harvester. The method gives a great saving both in time and in sample and ...
After making a sitting-drop plate, agarose solution was added to solidify the reservoir solution, and the plates were incubated upside down. Crystallization experiments with hen egg white lysozyme, thaumatin and glucose isomerase showed that the `upside-down sitting-drop' method could produce single ...
Crystallization and Preliminary X-Ray Analysis ofStreptococcus pneumoniaeHyaluronate Lyase A fully active 83-kDa truncated form of recombinant hyaluronate lyase from Streptococcus pneumoniae was crystallized by the hanging drop vapor diffusion me... MJ Jedrzejas,L Chantalat,RB Mewbourne - 《Journal of ...