(A) Venn diagram of total proteins identified in each TMT proteomics experiment. (B) Principal component analysis of Chinese hamster ovary (CHO) cells and hamster tissues. Clustering of overall protein intensity fold changes was calculated using the first and third principal components to show ...
Fig. 1. Schematic diagram of establishing the CRISPRa library screening platform in CHO-bsAb cells. (A) A landing pad master cell line (LP MCL) was created by sequential integrations of two landing pads into CHO-bsAb cells. The first landing pad harboring the mCherry gene and recombination ...
Fig. 5. Venn’s diagram representing the distribution of the number of differentially methylated regions-containing genes across the contrasts control (Ctrl) vs senescence (SEN), Ctrl vs senescence-bypass (SENbp), and SEN vs SENbp. The lists of genes for the region A, B, C, D, E, F,...
A flow diagram of this experiment is depicted in Figure 1. culture, the cells were grown to near confluence in a 75-cm2 flask. Thereafter, cells were subcultured at 80–90% confluence every 3 days at a dilution ratio of about 1:30, continuously for 6–7 weeks (up to 16th passage)....