The hammerhead ribozyme is one of the smallest of known catalytic RNAs. Its structure and mechanism have been examined using a broad range of approaches. Recently, crystal structures of the hammerhead have been reported. Within the crystal the hammerhead exists in a Y-shaped configuration in which...
Ribozyme: A clinical tool The hammerhead ribozyme was first discovered as a self-cleaving domain in the RNA genome of different plant viroids and virusoids [52]. Replication of these pathogens occurs by a rolling circle mechanism [53], in which the circular RNA genome is copied into long, mu...
The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage. We have solved the crystal structure of an all-RNA hammerhead ribozyme having a single 2'-O-methyl cytosine incorporated at the active site to prevent clea... WG Scott,JT Finch,A Klug ...
Hammerhead ribozyme Hammerhead Ribozyme Stylized rendering of the full-length hammerhead ribozyme RNA molecule Scientific classification Species: Catalytic RNA
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These results indicate that an unrecognized mechanism of post-tran-scriptional gene regulation involving association of discontinuous ribozyme sequences within an mRNA may be modulating the expression of several CLEC2 proteins that function in bone remodelling and the immune response of several mammals....
Naturally occurring hammerhead ribozymes were identified initially within RNA viruses and they act in cis during viral replication by the rolling circle mechanism (Symons 1989, 1992; Bratty et al. 1993). Hammerhead ribozymes have been engineered in such a way that they can act in trans to ...
In addition, a tRNA ligase-catalyzed reverse reaction is in a sense the most extreme example of a distinct mechanism for the reverse reaction. The proposal that at least some hammerhead ribozymes might possess a mechanistically distinct reverse reaction should be testable in that it predicts ...
A ligand-responsive hammerhead ribozyme is provided. In some embodiments, the ribozyme comprises: i. a first loop that has been replaced by an RNA aptamer that binds to a ligand; an
The hammerhead ribozyme has long been considered a prototype for understanding RNA catalysis, but discrepancies between the earlier crystal structures of a minimal hammerhead self-cleaving motif and various biochemical investigations frustrated attempt to understand hammerhead ribozyme catalysis in terms of str...