In its native form the enzyme is probably a homodimer with a relative molecular mass of the subunits of 63 600 (including the His-tag). Transformation of the pyrrolnitrin producer Pseudomonas chlororaphis ACN with a plasmid containing the tryptophan 6-halogenase gene lead to the formation of ...
This study reports the overexpression and purification of HalY with an N-terminal hexahistidine tag, followed by crystallization experiments and X-ray crystallographic analysis. HalY was purified as a monomer in solution and crystallized to give X-ray diffraction to a resolution of 1.7 Å. ...
Tag-encoded amplicon pyrosequencing was conducted with universal prokaryote primers PRK341F (CCT AYG GGR BGC ASC AG) and PRK806R (GGA CTA CNN GGG TAT CTA AT) to amplify an approximately 466 bp fragment of the 16S rRNA gene N comprising the V3 and V4 regions (Inceog˘lu et al., ...
This study reports the overexpression and purification of HalY with an N-terminal hexahistidine tag, followed by crystallization experiments and X-ray crystallographic analysis. HalY was purified as a monomer in solution and crystallized to give X-ray diffraction to a resolution of 1.7 Å. ...
Through the introduction of a His6-tag to both the N- and C-termini, the isolation yield of Rdc2 fromEscherichia coliusing Ni-NTA affinity chromatography was increased by three-fold.In vitroreaction of Rdc2 and a flavin reductase (Fre) with seven different hydroxyquinolines revealed that 3-...