After amplification with 1 microM methotrexate, a representative clone expressed about 15 pmol of P450 2E1/mg microsomal protein. Cells from a single 35-mm plate catalyzed the formation of 1.02 nmol 6-hydroxychlorzoxazone/10(6) cells/h or about 127 pmol/mg total cell protein/min. The enzyme...
·Strong balance sheet with access to £579 million of liquidity, net debt of £1.5 billion (Dec 2023: £1.5 billion) and EPRA loan-to-value ratio of 30 per cent (Dec 2023: 31 per cent) KEY FINANCIALS 1. Refer to note 2 'Performance Measures' on page 30. 2. Refer to note 1...
After amplification with 1 microM methotrexate, a representative clone expressed about 15 pmol of P450 2E1/mg microsomal protein. Cells from a single 35-mm plate catalyzed the formation of 1.02 nmol 6-hydroxychlorzoxazone/10(6) cells/h or about 127 pmol/mg total cell protein/min. The enzyme...
The inserts were then placed in the wells of a 24-well-plate containing 750 ml of DMEM-FBS (5%) and incubated at 37 °C for 6 h. After incubation, the cells on the top of the membrane were removed with a cotton swab. The cells migrated to the underside of the membrane were...
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The absorbance at 635 nm was then determined using a Tecan M200 Infinite plate reader. The rate of GmhA was determined by comparison to a phosphate standard curve ranging from 0–70 μM phosphate, and by subtracting the background rate from negative controls containing no GmhA. All ...
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Absorbance was recorded at 405 nm using a Sunrise plate-reader (TECAN). PBS/S/T (pH 7.4) or PBS/T was used as dilution and washing buffers, respectively. Complement ELISAs ELISAs were performed by capturing anti-NIP IgG1 variants (3000.0–23.4 ng/mL) on BSA-NIP(16) (diluted...
s (Sn2 sites, Wyckoff position 3a(0, 0, 0)) (Fig.1b) and another half of Sn atoms located between the Co–Sn bilayers (Sn1 sites, Wyckoff position 3b(0, 0, 1/2)), connecting adjacent Co–Sn2 layers. S atoms are located below and above the Co–Sn2 layers. For the plate-like...
The inserts were then placed in the wells of a 24-well-plate containing 750 ml of DMEM-FBS (5%) and incubated at 37 °C for 6 h. After incubation, the cells on the top of the membrane were removed with a cotton swab. The cells migrated to the underside of the membrane were fixed...