'daughter' strands, which requires the addition of new histones.To make things more complicated, one of the two DNA strands can be replicated only in short pieces - called Okazaki fragments - that are joined together afterwards, and so its replication lags behind that of the other strand (...
Origin licensing ensures precise once per cell cycle replication in eukaryotic cells. The Origin Recognition Complex, Cdc6 and Cdt1 load Mcm2-7 helicase (MCM) into a double hexamer, bound around duplex DNA. The complex formed by ORC-Cdc6 bound to duplex
Part of the DNA that full siblings inherit will be the exact same DNA from Mom and Dad, meaning that the siblings will match at the same location on their DNA on both Mom’s strand of DNA and Dad’s strand of DNA. These sections are called Fully Identical Regions, or FIR. Half sibli...
This protein plays a key role in HIV infection by facilitating the nuclear import of the pre-integration complex (PIC) that contains the viral DNA as well as several cellular and HIV proteins, including the integrase. The process of nuclear import is considered to be the bottleneck of the ...
She agreed to spit into tubes, first for an aunt/niece paternity test that revealed we were “86 percent likely” to be closely related. Later, we tested our whole-strand DNA, revealing that we indeed shared 25 percent of our genetic material — we were, without a doubt, aunt and niece...
GLnexus是由DNAnexus开发,用于可扩展的gVCF合并和联合变异(joint calling)要求群体测序项目,GL即genotype likelihood之意。 GATK作为变异检测金标准软件,缺点在于速度很慢。尽管在分析上游的比对、单样本HaplotypeCaller检测等环节已经有很多替代品,如Sentieon、Parabricks等,但下游joint calling这一步优化仍然有限。而这正是...
GLnexus是由DNAnexus开发,用于可扩展的gVCF合并和联合变异(joint calling)要求群体测序项目,GL即genotype likelihood之意。 GATK作为变异检测金标准软件,缺点在于速度很慢。尽管在分析上游的比对、单样本HaplotypeCaller检测等环节已经有很多替代品,如Sentieon、Parabricks等,但下游joint calling这一步优化仍然有限。而这正是...
A total amount of 2 mg of each pool of the plasmid DNA was used per injection per animal. Four and 8 days after the first and the last mMOG immunization, 100 mL anticoagulated blood was collected from each animal for the preparation of peripheral blood lymphocytes. The VHH encoding genes ...
of proteins are enzyme-medicated covalent alterations of protein sequence during which a chemical group can be added to a particular residue or sequence is cleaved at a specific location [ 1 ]. these modifications greatly expand the range of possible final forms of proteins that can be generated...
by delivering the fusion protein to the cell or by delivering a polynucleotide encoding the fusion protein to a cell, wherein the polynucleotide, if DNA, is transcribed, and an RNA molecule delivered to the cell or a transcript of a DNA molecule delivered to the cell is translated, to gener...