Functional analysis of genes involved in legume nodulation has been proven as successful for model legumes, viz., Medicago truncatula and Lotus japonicus, using transgenic hairy roots. Besides sharing some commo
PLANT genetic transformationLOTUS japonicusROOT diseasesAGROBACTERIUMRHIZOBIUMFor decades, Agrobacterium rhizogenes (now Rhizobium rhizogenes), the causative agent of hairy root disease, has been harnessed as an interkingdom DNA delivery tool for generating transgenic hairy roots on a...
Keywords: Agrobacterium strains; hairy root transformation; CRISPR/Cas; genome editing; vector construction; OsMac3; pKSEe401R 1. Introduction Interest in the manipulation of genes in the whole plant or its organs has increased since 1983 when several studies first reported the generation of ...
Leaves of Lotus japonicus cv. Miyakojima line MG-20 seedlings supplied with genistein produced isoprenylated isoflavonoid, wighteone. This observation led to the identification of novel prenyltransferase gene LjG6DT. The enzyme encoded by the gene worked only with genistein as a substrate and its ...
A. rhizogenes-mediated transformation has been realized in a plethora of species belonging to the family of Leguminosae, including both model plants, such as Lotus japonicus and Medicago truncatula [22,23], and cultivated species, such as Glycine max [24,25], Pisum sativum [26–28], Cicer ...