Progress in design of innovative bioreactors and process intensification for HR growth will allow successful industrial production of metabolites. This chapter will present advances in work on HR cultures related to the detoxification of pollutants, production of valuable metabolites, and their cultivation in large-scale intensified bioreacto...
obtained hairy root cultures of cannabis by inoculating the epidermis of hypocotyls withR. rhizogenes. As shown in their study, there are several factors (e.g.,Rhizobiumstrains,antibiotic,optical density(OD),cocultureperiod) that should be optimized for hairy root culture. In another study,Farag ...
Hairy roots (HRs) are differentiated cultures of transformed roots generated by the infection of wounded higher plants with Agrobacterium rhizogenes. This pathogen causes the HR disease leading to the neoplastic growth of roots that are characterized by
Expression of rol genes in planta modulates auxin homeostasis and induce hairy root initiation and proliferation in vitro; thus, hairy root cultures do not require the addition of phytohormones. Hairy roots are anatomically and metabolically similar to normal roots, and possess intact xylem and phloem...
cells show neoplastic growth that further differentiates to form “hairy roots.” These neoplastic roots possess high growth rates andgeneticstability. A higher level of secondary metabolites (in comparison with the naturally growing plant) can be produced by these genetically transformed root cultures....
Construction of plant expression vectors and hairy root cultivation. The SmJAZ3/SmJAZ9 gene or anti-SmJAZ3/anti-SmJAZ9 was amplified via PCR as indicated above. The vectors pCAMBIA1304 and pCAMBIA2300 were double-digested with Hind III and EcoR I. The smaller DNA fragment containing ...
stramoniumdiploid and tetraploid hairy root cultures weresubcultured more than one year before the presentinvestigation. Their chromosome numbers werechecked in squashed root tips.Cultivation of hairy rootD.stramoniumhairy roots used in the experi-ments were cultivated in hormone free liquid MSnutrient ...
求翻译:Hairy-root induction and cultivation in airlift bioreactor是什么意思?待解决 悬赏分:1 - 离问题结束还有 Hairy-root induction and cultivation in airlift bioreactor问题补充:匿名 2013-05-23 12:21:38 毛状根诱导和气升式生物反应器中的培养 匿名 2013-05-23 12:23:18 大闸-根入门和种植...
Hairy roots thus obtained were grown on solid MS medium or and in 1 L flasks containing 500 mL liquid culture shaken at 100 RPM. Figure 1 a: Expression cassette driving the production of human AChE-R ER in transgenic plants and hairy-root cultures. L, left border; R, right border; Ag...
Hairy roots are produced when the soil phytopathogen, Agrobacterium rhizogenes, infects a host plant. Just like normal roots, the hairy roots have the capacity to absorb target elements and produce valuable phytochemicals. Hairy roots have thus been expl