After labeling, cells were fixed and permeabilized. EdU Click reaction solution was then applied, and the cells were incubated in darkness for 30 min. DAPI was used for nuclear staining. Images were captured utilizing an Olympus FSX100 microscope. Cell migration assay The migratory capacity of ...
Cashmere, named as “soft gold”, derives from the secondary hair follicles (SHFs) of cashmere goat which is vital to Northwest China’s economy. The cytodifferentiation stage (E120), mirroring the complete hair follicle (HF) structure of adult goats and
Application of this labeling technique to the hair follicle led to the discovery in 1990 that the LRCs were mostly restricted to the bulge region, a specialized portion of the outer root sheath epithelium defined as the insertion site of the arrector pili muscle (Cotsarelis et al, 1990;Morris...
After labeling, cells were fixed and permeabilized. EdU Click reaction solution was then applied, and the cells were incubated in darkness for 30 min. DAPI was used for nuclear staining. Images were captured utilizing an Olympus FSX100 microscope. Cell migration assay The migratory capacity of ...
(Rice oligo microarray, 4 × 44 K). The cRNA was generated from RNA prepared separately from the four replicates of root-hair samples, following the manufacturer’s recommended protocols for the low-input quick amp labeling kit, one-color, (Agilent; 5190–2305). Afterward, 100 ng of ...
There is strong evidence that the cells responsible for the second phase of anagen are stem cells that reside in the bulge of the outer root sheath. 3H-thymidine or BrdU labeling revealed a population of slowly cycling (i.e., label-retaining) bulge cells in rodent and human hair follicles ...
or membranous CFP-labeled cells38. This system allows for the discrimination between the clonal progeny of individual cells derived from BSCs after recGDNF or vehicle treatment over time. After a single topical application of 1% RU486 to induce Confetti labeling, we wounded 2-month-of-age mice...
The cRNA was generated from RNA prepared separately from the four replicates of root-hair samples, following the manufacturer’s recommended protocols for the low-input quick amp labeling kit, one-color, (Agilent; 5190–2305). Afterward, 100 ng of the total RNA was transcribed to double-...
Activated caspase-3 labeling corresponded with hair-cell labeling (Fig. 7F,G), suggesting that activation of downstream cell death pathways in hair cells is not indirectly mediated by supporting-cell damage or death. These data reveal that ngn1morphant NM hair cells exposed to KA or NMDA ...
To verify that the exosomes derived from the DPCs can be transported to HFSCs, after labeling the DPCs with DiI these were co-cultured with HFSCs in 0.4 μm transwells. As a negative control, HFSCs were incubated in the recovered medium used as the final wash for DiI-labeled cells, and...