There is strong evidence that the cells responsible for the second phase of anagen are stem cells that reside in the bulge of the outer root sheath. 3H-thymidine or BrdU labeling revealed a population of slowly cycling (i.e., label-retaining) bulge cells in rodent and human hair follicles ...
The length and number of root hairs are regulated by genetic factors. Their formation follows one of three patterns: 1)random, any cell of the epidermis being capable of root-hair development; 2)alternative, morphologically different cells alternating along longitudinal epidermal cell files, with asy...
Application of this labeling technique to the hair follicle led to the discovery in 1990 that the LRCs were mostly restricted to the bulge region, a specialized portion of the outer root sheath epithelium defined as the insertion site of the arrector pili muscle (Cotsarelis et al, 1990;Morris...
The cRNA was generated from RNA prepared separately from the four replicates of root-hair samples, following the manufacturer’s recommended protocols for the low-input quick amp labeling kit, one-color, (Agilent; 5190–2305). Afterward, 100 ng of the total RNA was transcribed to double-...
1987. Isolation of root hairs from seedlings of Pisum sativum. Identi(R)cation of root hairs speci(R)c proteins by in situ labeling. Physiologia Plantarum 69, 129±136.ROHM, M. and D. WERNER: Isolation of root hairs from seedlings of Pisum sativum. Identification of root hair specific ...
After labeling, cells were fixed and permeabilized. EdU Click reaction solution was then applied, and the cells were incubated in darkness for 30 min. DAPI was used for nuclear staining. Images were captured utilizing an Olympus FSX100 microscope. Cell migration assay The migratory capacity of ...
Activated caspase-3 labeling corresponded with hair-cell labeling (Fig. 7F,G), suggesting that activation of downstream cell death pathways in hair cells is not indirectly mediated by supporting-cell damage or death. These data reveal that ngn1morphant NM hair cells exposed to KA or NMDA ...
Powers EM, Marshall JD (2011) Pulse labeling of dissolved 13C-carbonate into tree xylem: developing a new method to determine the fate of recently fixed photosynthate. Rapid Commun Mass Spectrom 25:33–40 CAS PubMed Google Scholar Rosell JA (2016) Bark thickness across the angiosperms: more...
or membranous CFP-labeled cells38. This system allows for the discrimination between the clonal progeny of individual cells derived from BSCs after recGDNF or vehicle treatment over time. After a single topical application of 1% RU486 to induce Confetti labeling, we wounded 2-month-of-age mice...
Using double immunofluorescence labeling we and others (Legg et al, in press) have found that MCSP colocalizes with β1 integrin bright cells within the tips of the dermal papillae. Moreover,Legg et al (in press) have shown that the colony forming efficiency of MCSP positive cells is ...