Surprisingly, substitution of the proximal residue Arg-124 with Phe, Ala, Gln or Glu also resulted in the six-coordinate adduct not being detected, showing that this proximal residue also modulates reactivity towards NO on the opposite haem face. In contrast, the R124L...
We previously reported that a 17.5-kDa haem-binding polypeptide accumulates in Escherichia coli K-12 mutants defective in an essential gene for cytochrome c assembly, ccmF, and speculated that this polypeptide is either CcmE or CcmG. The haem-containing polypeptide, which is associated with the ...
Andrew, et al.A two-faced molecule offers NO explanation: the proximal binding of nitric oxide to haem. Biochemical Society Transactions . 2003Lawson DM, Stevenson CE, Andrew CR, George SJ, Eady RR (2003) A two-faced molecule offers NO explanation: the proximal bind- ing of nitric oxide ...