Compared with the vaccine strains, there were no statistically significant of HA and NA genes and amino acid sequences of influenza A viruses in immunosuppressed and immunocompetent patients. However, the oseltamivir resistance substitution of NA-H275Y and R292K have been observed in immunosuppressed...
ZENG Qinghang, LIU Yang, SUN Minhua, HU Xinyu, XIE Zimin, ZHANG Minxia, YUAN Zhaoxia, LIAO Ming. Biological Characteristic of HA and NA Genes of H9N2 Avian Influenza Virus in Guangdong Province[J]. Guangdong Agricultural Sciences, 2023, 50(5): 103-111. 中文摘要【目的】 监测广东地区H9N2亚...
关键词:H1N1亚型猪流感;双重RT-PCR方法;HA基因;NA基因 中图分类号:S852.659.5文献标志码:A文章编号:1674-6422(2014)04-0029-06 DOUBLERT-PCRMETHODSFORDETECTIONOFHAANDNAGENESOF H1N1SUBTYPESWINEINFLUENZAVIRUS WENXiao-hui,CAIChun-mei,WEIWen-kang,ZHAIShao-lun,LVDian-hong,JIAChun-ling, YUANJie,HUANGZhong,...
摘要:将表达禽流感病毒H5HA及N1NA基因的重组禽痘病毒rFPV-HA-NA连续传代至25代,取第5、15及25代 重组病毒作为受检代次,进行外源基因的PCR扩增与测序,同时比较这3个代次重组禽痘病毒的免疫效力。结果 对各代次重组病毒DNA的模板进行PCR扩增,均能够获得HA和NA两个目的片段;经测序证明两个外源基因在 ...
A Simple Restriction Fragment Length Polymorphism-Based Strategy That Can Distinguish the Internal Genes of Human H1N1, H3N2, and H5N1 Influenza A Viruses A simple molecular technique for rapid genotyping was developed to monitor the internal gene composition of currently circulating influenza A viruses....
云南省2009~2014年甲型H1N1流感病毒HA及NA基因特征分析Analysis of HA and NAGenes of Influenza A H1N1 Virus in Yunnan Province during 2009 -2014 了解云南省2009~2014年甲型H1N1流感病毒的流行趋势,研究HA和NA基因进化特征.对云南省近6年来上报的流感监测病例数据进行病原谱总结,挑选出23株甲型H1N1流感毒株进行...
The prevalence and genetic characteristics of the HA&NA genes of seasonal influenza A(H3N2) influenza viruses in the City of Nanning in 2019 FAN Yun-yan，QIN Jian-min, OU Song-feng, TANG Hong-yang, SHI Jian (N a n n i n g C e n te r f o r D i sease C o ntrol a n...
为了建立适用于临床诊断的H1N1亚型猪流感病毒快速检测方法,本研究根据GenBank已登录的H1N1亚型猪流感病毒HA和NA基因序列设计RT-PCR扩增引物,以H1N1亚型猪流感病毒、H3N2亚型猪流感病毒、猪瘟病毒和猪繁殖与呼吸综合征病毒为试验对照,通过优化RT-PCR反应条件和反应体系,建立了H1N1亚型猪流感病毒HA和NA基因双重RT-PCR定型...
目的 了解山东省2022-2023流感监测年度分离的A(H1N1)pdm09亚型流感病毒血凝素(hemagglutinin,HA)和神经氨酸酶(neuraminidase,NA)基因的变异特征,为流感的防控提供科学依据.方法 从流感监测网络实验室分离的流感毒株中按地市随机选取14株A(H1N1)pdm09亚型流感毒株,以WHO推荐的当季疫苗株为参考进行全基因组测序,并采用...
代的H5N1病毒的主要抗原HA和NA的进化和变异情况进行了研究,并选择合 中山人学硕士学位论文中国禽流感病毒(H5NI)卧、NA基因的分予进化及克隆、表达研究 适的表达系统(毕赤酵母表达系统)对HA和NA蛋白进行了克隆和表达,为在 毕赤酵母系统中生产新型病毒样颗粒疫苗打下基础。