H3K27me3是核心组蛋白H3的第27个氨基酸赖氨酸的氨基(N)末端尾部发生的组蛋白甲基化,是最为常见的组蛋白甲基化修饰之一。H3K27me3是具有组蛋白甲酰基转移酶活性的多梳抑制复合体2 (Polycomb Repressive Complex 2,PRC2)的下游靶标,PRC2在干细胞多能性、体细胞分化和增殖中具有不同的作用,PRC2的酶亚基EZH1和...
是核心组蛋白H3的第27个氨基酸赖氨酸的氨基(N)末端尾部发生的,是最为常见的组蛋白甲基化修饰之一。H3K27me3是具有组蛋白甲酰基转移酶活性的多梳抑制复合体2(Polycomb Repressive Complex 2,PRC2)的下游靶标,PRC2在干细胞多能性、体细胞分化和增殖中具有不同的作用,PRC2的酶亚基EZH1和EZH2催化H3K27的甲基化,...
物种：Human, Mouse, Rat Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K27 antibody (A22006) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the...
ChIP assays were performed using human HeLa cells, the monoclonal antibody against H3K27me3 (cat. No. C15200181) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010051) on sheared chromatin from 1 million cells. A titration of the...
H3K27me3 Antibody (PA5-31817) in IF Immunofluorescent analysis of Tri-Methyl-Histone H3 mLys27 in paraformaldehyde-fixed A431 cells using a Tri-Methyl-Histone H3 mLys27 polyclonal antibody (Product # PA5-31817) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Produc...
H3K27me3 Antibody——EpiCypher新品推荐 H3K27me3是核心组蛋白H3的第27个氨基酸赖氨酸的氨基(N)末端尾部发生的组蛋白甲基化,是最为常见的组蛋白甲基化修饰之一。H3K27me3是具有组蛋白甲酰基转移酶活性的多梳抑制复合体2 (Polycomb Repressive Complex 2,PRC2)的下游靶标,PRC2在干细胞多能性、体细胞分化和增...
H3K27me3 Antibody (720069) in ICC/IF For immunofluorescence analysis HeLa cells were fixed and permeabilized for detection of H3K27me3 using Histone H3K27me3 Rabbit Polyclonal antibody (Product # 720069, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Seconda...
ChIP assays were performed using human HeLa cells treated with colcemid, the Diagenode antibody against H3K27me3S28p (Cat. No. C15410091) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010051), using sheared chromatin from 1 millio...
Antibody 抗原 rabbit immunized with the region of the histone H3 containing the trimethylated lysine 27 (H3K27me3) 偶联物 Unconjugated 形式 Liquid 浓度 1.6 mg/mL 纯化类型 Affinity chromatography 保存液 PBS 内含物 0.05% sodium azide, 0.05% ProClin 300 ...
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys27 is tri-methylated. Background The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of ch...