The following primary antibodies were used: acetyl-histone H3 (EMD Millipore; 06-599, 1:200), acetyl-histone H3 (Lys27) (Active Motif; 39133, 10 mg), and tri-methyl-histone H3 (Lys4) (Active Motif; 39159, 1:200). The chromatin/immune complexes were washed with a low-salt immune ...