用纯化的植物过氧化氢(H2O2)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入过氧化氢(H2O2),再与HRP标记的过氧化氢(H2O2)抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的过氧化氢(...
Figure 5.(a) Scheme of MFC setup; (b-e)H2O2production rate at the respective current density on a Co-N-C electrode with 0.1 mg Co-N-C cm-2 in O2-saturated 0.1 M KOH: (b) H2O2production rate normalized by ...
25、thedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensitytheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfatheresultisthesampleactualdensity.7Assayrange40ng/ml-1600ng/mlStorageandvalidity1. Storage2-8C.2. validitysixmon...
WT-EXAFS结果表明,Mo1/OSG-H中的Mo原子应原子分散在氧、硫掺杂的石墨烯骨架上,并且很可能在Mo-O和Mo-S部分的混合物中配位。 Figure 3. Electrocatalytic performance of Mo1/OSG-H sample for 2 e–ORR. a) ORR disk current...
Figure 5 (a) The charge difference density between g-C3N4 and ZIF-L (the yellow represents the electron accumulation area, and the light blue represents the electron dissipation area); (b) the planar averaged charge density difference along z-axis; (c)-(d) simulated stress and potential dist...
its practical application is challenged by the low-energy density, storability, and transportability of hydrogen gas4,5. To this end, photosynthesis of H2O2, an emerging liquid fuel and also a green oxidant, is attracting growing interests6. Among primary photosynthetic systems, including photovoltai...
(bottom row). Green arrow indicates a cell with nuclear FOXO1 and low levels of p53. Red arrow shows a cell with cytoplasmic FOXO1 and increased levels of p53. Experiments were repeated over 10 times with similar results.bDensity colored scatter plots (n ≥ 2000 cells) of the log of...
lots.Calculate Take the standard density as the horizontal the ODvalue for the vertical draw the standard curve on graphpaper Find out the corresponding density according to thesample OD value by the Sample curve multiplied by thedilution multiple or calculate ...
generation, following confluency, the cells were trypsinized using Gibco™ Trypsin–EDTA (Gibco, USA) and counted then seeded at a density of 2 × 104 cells/well in the 96-well agarose-coated plate and followed by centrifugation (swing bucket) at room temperature at 1000g for 30 min...
(ESP) charge density distributions of optimized PCN and Na-PCN; (h) and (i) Electronic structures of LUMO and HOMO of the optimized PCN and Na-PCN. The cyan and purple denominations represent electron and hole distribution...