H2B-RFP融合蛋白在观测肿瘤活细胞有丝分裂中的应用 作者:陈平波 墨青青 王蓓蓓 翁丹卉 吴鹏 陈刚组蛋白h2b红色荧光蛋白有丝分裂活体肿瘤细胞 摘要:目的:肿瘤细胞染色体分裂异常是导致肿瘤基因组不稳定的根本原因。这种不稳定性促进了肿瘤的进展和恶化。本文旨在探讨H2B—RFP融合蛋白能够更好地观测肿瘤活细胞有丝分裂过程...
MitoFlag(L-D)和 Hela细胞(带H2B-RFP红色荧光标记)共同孵育后在线粒体发现H2B存在,而加入H2B核定位序列(NLSH2B)后线粒体没有观察到H2B荧光信号,说明NLSH2B竞争性地与H2B-RFP、MitoFlag(L-D)相互作用,表明MitoFlag(L-D)可能通过NLS结构域...
RFP-LANA associated with GFP-fused histone H1 and H2B.Subhash C. VermaQiliang CaiEdward KreiderJie LuErle S. Robertson
HT-1080 human fibrosarcoma cells were labeled in the nucleus with histone H2B-GFP and with retroviral RFP in the cytoplasm. The nucleolus was visualized ... K Yamauchi,Y Meng,K Hayashi,... - 《Cell Cycle》 被引量: 23发表: 2007年 乳腺癌细胞休眠期至分裂间期染色质行为动力学特征分析 目的 ...
For labeling H2B-Cherry expressing cells, RFP AB was used (Table 2). 2.5. Immunocytochemistry Fixation and staining of adherent cells were performed as described in Wang et al., 2018. Embedding, sectioning and staining of 3D cell aggregates were performed according to Bastidas-Ponce et al., ...
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陈浩南目前担任布瑞尼(上海)教育技术有限公司法定代表人,同时担任布瑞尼(上海)教育技术有限公司总经理,执行董事;二、陈浩南投资情况:陈浩南目前是布瑞尼(上海)教育技术有限公司直接控股股东,持股比例为66%;目前陈浩南投资布瑞尼(上海)教育技术有限公司最终收益股份为66%;三、陈浩南的商业合作伙伴:基于公开数据展示,陈...
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We show that Rhp6 is a component of the multisubunit protein complex (termed HULC) that also contains two RING finger proteins Rfp1 and Rfp2, sharing homology with budding yeast Bre1 protein and a unique serine-rich protein Shf1. HULC is required for ubiquitination of histone H2B at lysine...
Hair Follicle Morphogenesis and Differential Expression of Lef1-RFP and K14-H2BGFP in All Cells of the Mature FollicleMichael RendlLisa LewisElaine Fuchs