This study is to identify putative LSC markers by analyzing label-retaining cells (LRCs) from limbus of H2B-GFP mice at single-cell level. Methods : H2B-GFP mice express H2B-GFP under doxycycline (dox). Mice were fed with dox for 4 weeks, followed by 2 rounds of wound healing on ...
Hematopoietic stem cell response of wild-type, dox-treated TET-GFP and H2B-GFP mice to 5-FU.Grant, A. ChallenMargaret, A. Goodell
(e) Lung disseminated cells in mice injected with either MDA-MB-468 or MCF7-RAS cells, quantified by image analysis of GFP fluorescence in lungs (A.U., arbitrary units). *Po0.05. Error bars, S.E. (f) Representative images of metastatic cells (green) in lungs of tumor bearing mice, ...
RE-IIBP Associates with RNF20 to Synergistically Activate MEIS1 Transcription. To gain a better understanding of the proteins that interact with RE-IIBP, GFP-RE-IIBP was overexpressed in 293T cells and GFP affinity purification was performed. The immunoprecipitated proteins were analyzed by ...
Osteosarcoma U2-OS cells were transfected with GFP constructs 24 h before micro-irradiation, and incubated with 10 mg ml À 1 Hoechst 34580 for 30 min before irradiation. Cells were micro-irradiated with a 405 nm UV-laser at a dose of 0.22 mJ mm À 2 (ref. 42), and time-lapse ...
To characterize B6-Dct-H2BGFP mice, we confirmed not only the expression of GFP in all melanocyte lineage cells, but also doxycycline regulation of GFP expression. Furthermore, ex vivo culture of the McSC subsets isolated by fluorescence-activated cell sorting (FACS) showed the propensity of ...
Uniform Loss of Label in Adult β Cells Following Pulse–Chase with Pdx1-tTA; tetO-H2BGFP and Rosa26-rtTA; tetO-H2BGFP MiceKristen, BrennandDanwei, HuangfuDoug, Melton
To begin testing this hypothesis, we used an inducible H2B-GFP transgenic mouse model to identify slow-cycling, label-retaining cells (LRCs) within the uterus. H2B-GFP expression was induced embryonically to label all cells within the uterus. As the mice aged, GFP label was lost in highly ...
Localization and characterization of thymic label-retaining cells in K5tTA;TetO-H2BGFP mice.Masako OsadaVaran J. SinghKenmin WuDerek B. Sant’AngeloMark Pezzano
Changes in H2BGFP expression within TECs in K5tTA;TetO-H2BGFP transgenic mice following a time course of Dox feeding.Masako OsadaVaran J. SinghKenmin WuDerek B. Sant’AngeloMark Pezzano