Bernstein. The mH2A1.1-mCherry constructs expressing various fragments of mH2A1.1 were generated from the retroviral construct pLPC-mH2A1.1-mCherry by using a PCR-based gene deletion strategy based on the domain structure of mH2A1.1. dC, deletion of the macro domain; dL, deletion of the ...
.B and PCNA-mCherry were obtained every 5 min (seeSupplementary Movie S1). The elapsed time (hh:mm) from the start of recording is shown on each panel. During mid- to late-S phase, GFP-H2A.B becomes clearly concentrated in PCNA foci, where DNA replication occurs. Bars, 10 μm...
(Cell Signalling 5246) at 1:500 dilution, anti-EED (a kind gift from A. Otte) at 1:100 dilution, anti-JARID2 (Novus Biologicals NB100-2214) at 1:500 dilution, H2AK119u1 (NEB 8240) at 1:500 dilution, anti-FLAG (Sigma M2 F1804 and F7425) at 1:500 dilution, anti-mCherry (...
Genomic stability is critical for normal cellular function and its deregulation is a universal hallmark of cancer. Here we outline a previously undescribed role of COMMD4 in maintaining genomic stability, by regulation of chromatin remodelling at sites o