结论 细胞热损伤后可发生S期阻滞, 阻滞期间因复制继续、DNA损伤修复抑制而延迟形成的致死性DNA双链断裂是S期细胞热敏感的可能原因。 关键词: H1299细胞 热损伤 细胞周期阻滞 DNA双链断裂 Delayed DNA double-strand break in S-phase H1299 cells after thermal damage ...
The results showed the highest numbers of apoptotic cells in both H460 and H1299 cells following the combined treatment regardless of p53 status in comparison with either cisplatin or Avp53 alone. The expression levels of p53, p21, Bax and ICE were examined to understand a possible cellular ...
本实验以我们培养的A549、H1299细胞为实验对象,主要观察不同温度加热处理对P53表达情况不同的两种肺癌细胞生长、细胞周期和凋亡的影响有无差异,并初步探讨热疗促肺癌细胞凋亡的机制。 1材料和方法 1.1材料 人肺癌A549、H1299细胞株由苏州大学医学院分子生物实验室提供。RPMI1640培养基(Gibco),小牛血清(杭州四季青),...
hnRNP K suppresses apoptosis independent of p53 status by maintaining high levels of endogenous caspase inhibitors. Carcinogenesis, 2013; 34, 1458-67. doi: 10.1093/carcin/bgt085 [22] Choi HS, Hwang CK, Song KY, et al. Poly(C)-binding proteins as transcriptional regulators of gene expression....
Simvastatin induces apoptosis in human breast cancer cells: p53 and estrogen receptor independent pathway requiring signalling through JNK 2007, Cancer Letters Citation Excerpt : Moreover, deactivation of JNK also has been mentioned in clinical studies in prostate [28] and colon [29] cancers. A rece...
It is well known that p53 is a tumor suppressor and can also regulate the metabolic pathways of cancer cells. RBL2/DREAM-mediated Aurora kinase A/B pathway inhibition in p53 WT NSCLC can increase the radiosensitivity of NSCLC [35]. On the other hand, p53 is an important checkpoint in the...
The oxidative stress status, extent of DNA damage, expression of p53, p73, mdm2, bcl-2, and bax at the mRNA and protein levels, and the percentages of apoptosis and/or necrosis were assessed. In the two BaP-treated cell lines, we observed increased malondialdehyde (MDA) formation and ...
p53 status affects the rate of the onset but not the overall extent of doxorubicin-induced cell death in rat-1 fibroblasts constitutively expressing c-Myc. Cancer Res. 1997, 57, 176–182. [Google Scholar] [PubMed] Tannock, I.F.; Lee, C. Evidence against apoptosis as a major mechanism ...
p53 status affects the rate of the onset but not the overall extent of doxorubicin-induced cell death in rat-1 fibroblasts constitutively expressing c-Myc. Cancer Res. 1997, 57, 176–182. [Google Scholar] [PubMed] Tannock, I.F.; Lee, C. Evidence against apoptosis as a major mechanism ...
Likewise, human non-small-cell lung cancer (NSCLC) H1299 (p53-null) cell is more sensitive to curcumin than A549 (p53-wt) cells [13]. In that work, the apoptotic molecules p53, bcl-2, and bcl-XL were examined, however, less is known about the link between DDR pathway heterogeneity ...