This semi-constant-time HMSQC-HA experiment combines the rapidity and convenience of the recently introduced CT-HMQC-HA scheme (Postingl and Otting, J. Biomol. NMR 12, 319-324 (1998)) with the high resolution and robustness of the HSQC experiment. The proposed method is demonstrated for the...
The structure was determined by the HSQC and HMBC analyses. Pale yellow oil, 9.7 mg, 0.214 mmol, 11% isolated yield; 1H NMR (CDCl3, 400 MHz) δ 8.08 (d, J = 8.0 Hz, 1H, Hh), 7.48 (td, J = 7.5, 1.3 Hz, 1H, Hg), 7.31 (td, J = 7.7, 1.3 Hz, 1H, Hf), 7.28–7.22 ...
15N−1H HSQC NMR spectra reveal that the local conformations of the two active sites in homodimeric GlxI are initially asymmetric in the apo-form, resulting in functional differentiation, wherein only one active site binds to the Ni2+ ion, and another active site is observed to be more ...
The 1 H and 13 C NMR resonances of 16 purine glucosides were assigned by a combination of one- and two-dimensional NMR experiments, including gs-COSY, gs-HSQC, and gs-HMBC, in order to characterize the effect of substituent and the position of glucose unit on the NMR chemical shifts. I...
H–H COSY, HMBC, and HSQC spectra of silylated HOBB were recorded. Biosynthesis and preparation of HOBB Biosynthesis was conducted with 200 mL of the bac- terial culture in a 1-L flask under the best conditions determined from all trials. Two strategies were used for biosynthesis. ...
Fast Assignment of 15N-HSQC Peaks using High-Resolution 3D HNcocaNH Experiments with Non-Uniform Sampling We describe an efficient NMR triple resonance approach for fast assignment of backbone amide resonance peaks in the 15 N- HSQC spectrum. The exceptionally... ZYJ Sun,DP Frueh,P Selenko,.....
in isotopically enriched proteins - Hu, Bax - 1997 () Citation Context ...re measured using HSQC-NOESY-TROSY experiments with a mixing time of 150 ms... HA Bax - 《Journal of Biomolecular Nmr》 被引量: 54发表: 1997年 加载更多来源...
C−H∙∙∙π and N−H∙∙∙π interactions can have an important contribution for protein stability. However, direct measurements of these interactions in proteins are rarely reported. In this work, we combined the mutant cycle experiments and molecular dynamics (MD) simulations to ch...
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