RNA was extracted from 5.5 Â 106 cells using the RNeasy RNA extraction kit (Qiagen, Chadstone, VIC, Australia), as per the manufacturer's instructions. In all, 1.5 mg of RNA was reverse transcribed using H-HLMV (Promega, South Sydney, NSW, Australia) and random primers (Promega, ...
hypotonic PI buffer staining and flow cytometric analysis. The percentage of cells in S-phase was determined using the cell-cycle analysis software ModFit. Results indicate means±S.E.M. of seven independent clones in three independent experiments. (d) Cells arrest in G1 after Hoxb8 downregulatio...