E. coliC600 using a double agar layer approach. To quantify bacteria, stool was frozen at −80 °C within 30 min of excretion and then immediately prior to analysis, thawed at room temperature, resuspended into 1 mL of PBS with vortexing for 10 min at 4 °C, and then ...
age [37] (Table2). In a recent metabolomics-based approach, stool samples from children aged 4 to 7 years with asthma were compared to healthy children, with a focus on gut metabolites such as amino acids or butyrate [38]. Taxonomic classification showed that among gut bacteria, the phylaF...
The researchers sequenced regions of microbial DNA from the stool samples to identify different types of bacteria. Overall, the diversity of types of bacteria was greatly reduced and there were fewer bacterial species known to be anti-inflammatory in ME/CFS patients compared with healthy people, an...
The genomes of bacteria derived from the gut microbiome contain several pathways that mediate contact-dependent interbacterial antagonism1,2,3. Many members of the Gram-negative order Bacteroidales encode the type VI secretion system (T6SS), which facilitates the delivery of toxic effector proteins ...
After preliminary screening (bacteria suspension) and re-screening (bacteria suspension, extracellular substance, intracellular substance, and bacteria fragment), a strain with strong antioxidant activity, named Lactobacillus plantarum 124 (LP124), was finally screened in vitro. The antioxidant screening ...
s health. In addition, many active consortium projects have generated many reference datasets available for large-scale retrospective research. However, a comprehensive monitoring framework that analyzes health status and quantitatively present bacteria-to-health contribution has not been thoroughly ...
To the best of our knowledge, this is the first study to discover gut microbiota dysbiosis among gallstone patients, the presence of which may be a key contributor to the complex bacteria community assembly linked with the presence of cholesterol gallstones. Likewise, this study also provides the...
The efficacy of TGD was tested by weekly stool culture. When persistent growth of aerobic gram-negative bacteria or yeasts was observed, additional non-absorbable antimicrobials were added based on susceptibility testing. Recolonization of the intestine after TGD was aided by the oral administration of...
Using humanized mice that were colonized with bacteria from the faeces of patients with alcoholic hepatitis, we investigated the therapeutic effects of bacteriophages that target cytolytic E. faecalis. We found that these bacteriophages decrease cytolysin in the liver and abolish ethanol-induced liver ...
probiotic/placebo supplement visit 4 and wash-out visit 5 in both groups. The reference gene used for relative qPCR was the 16S rRNA gene that targeted all bacteria. A Kruskal-Wallis test of One-way ANOVA (nonparametric) using Dunnett’s multiple comparisons analysis showed that there was an ...