实施例2:筛选导向化合物 剂量依赖性增殖抑制通过celltiterglo在两种白血病细胞系sem和p12中对选自自最初的电脑模拟筛选的100种化合物进行增殖抑制分析。将用于增殖分析的细胞沉淀并再悬浮。然后将细胞悬液分开,以所需的浓度加入要测试的化合物,随后进行平板培养。在孵育期间之后,加入celltiterglo试剂(promegacelltiter水相非...
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Main KRAS, NRAS and HRAS proteins hydrolyse GTP to GDP and control multiple cellular functions by cycling between an active (GTP bound) and an inactive (GDP bound) state3,4,5,6. These enzymes have a weak intrinsic GTPase activity, which is physiologically enhanced by GTPase-activating proteins...
Celltiter-glo or clonogenic assay were performed to evaluate drug/RT responses. IHC was used to detect protein expression. Animal models of glioblastoma and normal tissue (irradiation of GI system) were used to assess DNA repair and treatment responses in vivo.Results: Pharmacogenomic inhibition of ...
简单的采样探头来了，首先是MiniMonitor，金门计，Aqua-Glo，油底壳分离器 以及其他独特而有用的产品。一开始，GTP有 零件在外面制造，并在内部组装，但这发展迅速 进入车间运营，因为霍华德拥有广泛的机械车间和工程经验。如今，GAMMON产品经销覆盖全球每个国家，大部分是本地 小型专业企业。GAMMON对质量的承诺仍然在...
The CMS322GLO is LED Lenses Frosted Sealed Lens GLO Series 5mm manufactured by VCC. is part of the Optics - LEDs, Lamps - Lenses, , and with support for LED Lenses Frosted Sealed Lens GLO Series 5mm. 技术参数 Product LED Lenses Diameter 7.62 mm Material Polycarbonate (PC) Color...
The intrinsic GTPase activity of EsuDRG1 and HsaDRG1 recombinant proteins was measured using the GTPase-Glo Assay (Promega) according to the manufacturer’s guidelines. For optimization, the purified proteins were serially diluted in a GTPase/GAP buffer containing 2 µM GTP, and the assay was...
下载源码后,解压并进入xduugtp-main文件夹。 运行如下命令得到文档类文件及相应的示例文件: xelatex xduugtp.ins 运行如下命令得到文档类手册: xelatex xduugtp.dtx makeindex -s gind.ist -o xduugtp.ind xduugtp.idx makeindex -s gglo.ist -o xduugtp.gls xduugtp.glo xelatex xduugtp.dtx xelatex...
The present invention relates to compounds of Formula (I) or pharmaceutically acceptable salts or solvates thereof: It further discloses a pharmaceutical composition comprising the compounds of Formula (I) and their uses, in particular in the treatment of diseases or disorders associated to increased ...
CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7573 Active Ras Pull-Down and Detection Kit ThermoFisher Scientific Cat#16117 RNALater Qiagen Cat#76104 RNeasy Plus Mini kit Qiagen Cat#74136 RNeasy Mini kit Qiagen Cat#74106 On-column DNase digestion Qiagen Cat#79254 Lipofectamine RNAiM...