Flow rate (100 nl/min) was regulated by a syringe pump (World Precision Instruments). Following virus injection, skin adhesive (3M vetbond) was applied to close the incision site. General analgesia (Buprenorphine SR, 0.05 mg/kg) was injected subcutaneously and mice were monitored until they...
To generate cytosol fractions immunodepleted of specific PtdIns(3)P binding proteins, cytosol fractions prepared from HeLa cells were incubated with primary antibody against the respective proteins or control IgG overnight at 4 °C. Protein A Sepharose Fast flow beads (Cytiva) were added to remo...
J Cereb Blood Flow Metab 2002, 22:767-773. 32. Morita T: Heme oxygenase and atherosclerosis. Arterioscler Thromb Vasc Biol 2005, 25:1786-1795. 33. Berg A, Johns J, Baumstark M, Keul J: HDL-cholesterol (HDL-C) changes during and after intensive long-lasting exercise. Int J Sports Med...
The results in Figure 4 show that expression of wild-type hPAK1 (lane 3) or kinase-inactive hPAK1K299A (lane 4) in COS-7 cells had little effect on the kinase activity of JNK1, as compared to the basal JNK1 activity seen in transfections with vector alone (lane 2). In contrast, ...
Kinetic analyses of binding between Rab14 and RCP from stopped-flow fluorescence. Association (kon) and dissociation (koff) rate constants are shown, along with the derived dissociation constant (Kd). The RCP variants are as follows: RCPNF, S580N/S582F, residues 559–649; RCPEE, S580E/S58...
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(Applied Biosystems). Subsequently, the proteins were eluted by an Agilent 1100 binary pump system with the following gradient: 5–100% B in 10 min at a flow rate of 0.3 mL/min (A: 0.1% formic acid in water; B: 0.1% formic acid in 80% acetonitrile/water) and sprayed into a...
G EGFR expression on the surface of RAW264.7 was analyzed by flow cytometry. H Percentage of EGFR-positive RAW264.7 is shown (n = 3). I Mean fluorescence intensity (MFI) is shown (n = 3). J Cell lysates from the indicated Rab7a mutant RAW264.7 cells followed by LPS treatment...
Calcium mobilization was determined by the intensity ratio of Fluo-3/Fura Red fluorescence over time as recorded by flow cytometry. RESULTS Following T Cell Activation, Vav1 and Ly-GDI Accumulate in F-actin-rich Membrane Extensions That Protrude in the Contact Zone with the APC Upon stimulation ...
According to a general laparectomy method, the abdomen of the sows was opened by making a vertical incision about 7 cm long in the middle of the abdomen, while not allowing blood to flow into the inside of the abdomen. The ovary, oviduct and uterus were drawn to the opened region of ...