结论1.GSTP1(I105V)、RRM1(T-524C、C-37A)与晚期NSCLC患者对GP方案化疗的敏感性有关;2.GSTP1(I105V)位点AG及GG基因型与RRM1(T-524C、C-37A)位点CA/TC基因型的PFS更长。3.GSTP1(I105V)、RRM1(T-524C、C-37A)与OS无明显相关性。 展开 ...
实施例二引物的特异性 将本发明提供的引物于UCSC中进行Blasting,XRCC1_R399Q引物扩增片 段位于 chrl9: 44055651-44055888,长度为 238bp ;ERCC1_C118T 引物扩增片段 位于 chrl9: 45923504-45923722,长度为 219bp ;ERCC2_L751Q 引物扩增片段位 于 chrl9: 45854837+45855007,长度为 171bp ;GSTP1_I105V 引物...
目的 研究GSTM1,GSTT1和GSTP1基因多态性对多环芳烃接触工人尿中1-羟基芘(1-OHP)水平的影响.方法 分别选取2个炼焦厂共447名多环芳烃职业接触工人(接触组)和某线材厂220名非职业接触工人(对照组)作为研究对象,采用高效液相色谱法测定尿中1-OHP水平,采用线性回归统计模型分析GSTM1和GSTT1缺失型...
引物构成如下:PRDX1:5'-CGCGAGATCCCTACTGGCTA-3'和5'-TCCCAACACAAGTCGCAGAA-3';GSTP1:5'-CTATGGGAAGGACCAGCAGG-3'和5'-TGGTCTCCCACAATG-3';GPX1:5'-CAGTCGGTGTATGCCTTCTCG-3'和5'-GAGGGACGCCACATTCTCG-3';β-actin:5'-AGAGCTACGAGCTGC-3'和5'-AGCACTGTGTTGGCG-3';④qRT-PCR分析:分别...
catalytic efficiency for the diol epoxides of polycyclic aromatic hydrocarbons that is seven-fold higher than the isoenzymes having Ile105. Furthermore, theGSTP1GG genotype was found to be 2–3 times less stable than the AA genotype and was associated with a higher hydrophilic DNA adduct level...
The MST of patients with the GSTP1 AA genotype was 76.7 months, while the MST of patients with the AG or GG genotype was 40.4 and 23.3 months, respectively (the Log-rank P = 0.002) (Fig. 1A). Furthermore, a significant difference in the MST of patients with the AA ...
The relationship between GSTP1 I105V polymorphism and COPD: a reappraisal. Am J Respir Crit Care Med. 2010;181:763–765. doi:10.1164/ajrccm.181.7.763 21. Yan F, Chen C, Jing J, et al. Association between polymorphism of glutathione S-transferase P1 and chronic obstructive pulmonary ...
The mRNA expression of GSTP1 in PBMC of the patients with AG/GG genotypes were significantly higher than those patients with AA genotype (P<0.01). Conclusion: GSTP1 I105V genetic variation influences the recurrence risk and prognosis of CRC patients r...
结果显示GSTP1基因编码的I105V位点AA型98例,AG型32例,GG型3例,其与患者预后密切相关;随访患者复发及预后情况,显示AA基因型DFS,OS为(5.72±0.42),(7.03±1.02)年,高于AG/GG基因型的(3.98±0.31),(4.52±0.63)年,差异有统计学意义(P<0.05).AG/GG基因型是患者OS的独立影响因素;I105V位点AG/GG基因型外周...
基因多态性分析显示,位于GSTP-1基因编码区域的I105V位点和预后相关.I105V位点在研究人群中的分布频率:AA型135例(69.23%),AG型56例(28.72%),GG型4例(2.05%),最小等位基因频率为0.16,该位点基因型分布频率符合HardyWeinberg平衡(P>0.05).对基因型患者进行复发风险和预后的分析发现,AA基因型和AG/GG基因型患者的...