Epitope-tagged Protein Purification Reduced glutathione (GSH), when immobilized as a ligand to agarose or other chromatography supports, enables high-yield and high-quality purification of recombinant proteins expressed as fusions with g...
Glutathione-Superflow Resin allows rapid affinity purification of GST-tagged proteins for batch, gravity flow, and FPLC applications.
The scale of purification of GST-tagged proteins is dependent on the amount of protein in the preparation. A column size and total binding capacity should be chosen to approximately match the amount of protein to be purified. Very few non-tagged proteins are usually retained on the resin if ...
将还原型谷胱甘肽 (GSH) 固定后作为琼脂糖或其他色谱载体的配体时,可对表达为谷胱甘肽 S 转移酶 (GST) 融合的重组蛋白进行高得率和高质量纯化。GST 融合蛋白纯化具有高得率,因为12原子 GSH 连接子极大程度减小了空间位阻。我们提供多种形式的谷胱甘肽磁珠...
将还原型谷胱甘肽 (GSH) 固定后作为琼脂糖或其他色谱载体的配体时,可对表达为谷胱甘肽 S 转移酶 (GST) 融合的重组蛋白进行高得率和高质量纯化。GST 融合蛋白纯化具有高得率,因为12原子 GSH 连接子极大程度减小了空间位阻。我们提供多种形式的谷胱甘肽...
Glutathione-S-transferase (GST) tagged proteins have variety of applications including purification of fusion proteins. The GST pull-down assay is an in vitro method used to determine a physical interaction between a GST tagged probe protein to an unknown or a known target protein. The procedure ...
GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of ...
Amicon® Pro Affinity Concentration Kit - GST Purification of GST-tagged recombinant proteins.Catalog Nos. ACR5000GS, ACK5003GS, ACK5010GS, ACK5030GS,ACK5050GS, ACK5100GS FOR RESEARCH USE ONLY Not for use in diagnostic procedures.USA & Canada Phone: +1(800) 437-7500 Fax: +1 (951) ...
This protocol describes the purification of recombinant proteins fused to glutathione S-transferase (GST, GST-tagged proteins) by Glutathione Affinity purification. The GST tag frequently increases the solubility of the fused protein of interest and thus enables its purification and subsequent functional ...
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.